Sequences from confirmation amplicon


Confirmation sequences for line 554B11 and hit At5g41250 are shown.
Line ID 554B11
Locus At5g41250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16501250
Primers used for PCR 23xp - 8474, show primer details
   
Sequence Name 47-K119444-0022-554-B11C-23xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 23xp: 5'-ATATCAATATAGCGTGACTGCGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K119444-0022-554-B11C-23xp
GTGTTTCGATACAAAGGAAGCAACTTTGTGTTAAAGGGAACCTCTCAAATAGCTTAAGTC
TGCCATCAACAATAGCTGGAACTTTCCCCATTGGGTTAATTTCTGAGAAACAATGAATAC
ACACTTTAAGATCAAATCTCACTTTAAAGACTAATTCAAGCTTCAACTTTTATCCAAATC
CGAAATCACCCATGTCCAAAAAAAACTATTTATACGAACCTTTGAATTCAGGGGATAACT
GTTGACGTTTCCCCAAGGAAATCAAAATCTCGTCGAATTGGATTTCGTTAACCCTTCACA
CAAAAAGTAGGTCATGTATAAGAGAACAAACAAAAAGGCTGAAATCGCGAATTAACAAGG
AAGAAGAAGAAGGAGTTTACTTGCAGAATATGAGGACAGCACGAGATGGCTGTGACATCC
TATCCGCATACACTTTGAGCTTCATCTTCTTCTTCCTCCGATTTTGTGTATCGGAAAAAG
TTTTCAGAACACTGATGAACAAACAGAACGGTGATAATCTGGGGAAGAACCAAATTAGCT
TTGCTGTTTCCACAATAGAGACTAAAACGCCGTGCCGTTTTTCTAGACTTCTCTTAACCG
GCTGGTTAGACCAAATGCTCCTTCTTCAGTCTTCACGAATCAAATCATAACCGGTCCTTA
ACCAGCTCCAGTTGAGATCACAAATATATTTTATTTTCTGTTCTTTGAAAGTTTGAAACA
AATACATCATACCAAACTACAAAATTTATTTTATATTTATCGTATTTGTTCATCAAATAA
ACAAATAAGAATGGACCGGAAGCAATCATAATGATAATCACATTCATGAAGCGCAACATT
ACAAAAAATGTTATACCACTGACCTGTTGATTTTCTTAAGTAAAAGCCATTGACCATCAA
AAGCATCATATTCAATTGTA
   
Sequence Name 15-K119443-0022-554-B11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119443-0022-554-B11C-8409
TCGGAATGAGCATTTACATTGAATATGATGCTTTTGATGGTCAATGGCTTTTACTTAAGA
AAATCAACAGGTCAGTGGTATAACATTTTTTGTAATGTTTGCGCTCATGAATGTGATTAT
CATTATGATTTGCTCCGGTCCATTCTTATTTGTTTATTTGATGAACAAATACGATAAATA
TAAAATAAATTTTGTAGTTTGGTATGATGTATTTGTTTCAAACTTTCAAAGAACAGAAAA
TAAAATATATTTGTGATCTCAACTGGAGCTGGTTAAGGACCGGTTATGATTTGATTCGTG
AAGACTGAAGAAGGAGCATTTGGTCTAACCAGCCGGTTAAGAGAAGTCTAGAAAAACGGC
ACGGCGTTTTAGTCTCTATTGTGGAAACAGCAAAGCTAATTTGGTTCTTCCCCAGATTAT
CACCGTTCTGTTTGTTCATCAGTGTTCTGAAAACTTTTTCCGATACACAAAATCGGAGGA
AGAAGAAGATGAAGCTCAAAGTGTATGCGGATAGGATGTCACAGCCATCTCGTGCTGTCC
TCATATTCTGCAAGTAAACTCCTTCTTCTTCTTCCTTGTTAATTCGCGATTTCAGCCTTT
TTGTTTGTTCTCTTATACATGACCTACTTTTTGTGTGAAGGGTTAACGAAATCCAATTCG
ACGAGATTTTGATTTCCTTGGGGAAACGTCAACAGTTATCCCCTGAATTCAAAGGTTCGT
ATAAATAGTTTTTTTTGGACATGGGTGATTTCGGATTTGGATAAAAGTTGAAGCTTGAAT
TAGTCTTTAAAGTGAGATTTGATCTTAAAGTGTGTATTCATTGTTTCTCAGAAATTAACC
CAATGGGGAAAGTTCCAGCTATTGTTGATGGCAGACTTAAGCTATTTGAGAGGTTCCTTT
TACACAAAGTTGCTTCCTTTGTATCGAAACACCATTGTTTAT

Last Updated on 06.12.2018 16:11