Sequences from confirmation amplicon


Confirmation sequences for line 554C06 and hit At3g49690 are shown.
Line ID 554C06
Locus At3g49690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18427607
Primers used for PCR CP50 - 8474, show primer details
   
Sequence Name 28-K031008-022-554-C06C-8409
GenBank Accession LN498721 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K031008-022-554-C06C-8409
TTTCTAATTCCCTATTGTATAGTATTTACTTATGTTGAATTAATAAATTAAAACATTTAA
ATTTCCCTAAAGTTACTTTTTGATTTTTGATTTTCTATTTTTATTACAACTTTCCTACTT
TTTGACATCTTTTCTTACAAAGACTTTTTGACATCTCCCCCTCCAGCTATTATATAGTCT
CCTTTTTTCTTTTCTGCATTCACATAATATTTCCCCTATATAGTTTACACAACATCATAC
CCACCAACATATATAATCTTGATCATAGAGAGATAAACAGAGGCCGCTATCAAGAACAAG
ACTAAGAACAAGACTTCACTAGGAGTACAAGTATGGGAAGAGCACCGTGTTGTGACAAAG
CAAACGTGAAGAAAGGGCCTTGGTCTCCTGAGGAAGATGCAAAACTCAAATCTTACATTG
AAAATAGTGGCACCGGAGGCAATTGGATCGCTTTGCCTCAAAAGATTGGTATTAATAAAT
TTATAGTCACTCTTTTTCATATAAATTTGATAATTCTCTCTTGATCTATCTCTTTTTTTG
TGAAGAAGTATTGATAACTAAAGATGCTTTTTGTTTATATGATTATTTCTTGGTTGTTTT
AAGGTTTAAAGAGATGTGGAAGNAGTTGC
   
Sequence Name 29-K031008-022-554-C06C-CP50
GenBank Accession LN499168 [GenBank]
Sequencing primer gene specific primer, CP50: 5'-TGCAACTCTTTCCACATCTCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K031008-022-554-C06C-CP50
ATATCATATAACAAAAAGCATCTTTAGTTATCAATACTCTCTTCACAAAAAAAGAGATAG
ATCAAGAGAGAATTATCAAATTTATATGAAAAAGAGTGACTATAAATTTATTAATACCAA
TCTTTTGAGGCAAAGCGATCCAATTGCCTCCGGTGCCACTATTTTCAATGTAAGATTTGA
GTTTTGCATCTTCCTCAGGAGACCAAGGCCCTTTCTTCACGTTTGCTTTGTCACAACACG
GTGCTCTTCCCATACTTGTACTCCTAGTGAAGTCTTGTTCTTAGTCTTGTTCTTGATAGC
GGCCTCTGTTTATCTCTCTATGATCAAGATTATATATGTTGGTGGGTATGATGTTGTGTA
AACTATATAGGGGAAATATTATGTGAATGCAGAAAAGAAAAAAGGAGACTATATAATAGC
TGGAGGGGGAGATGTCAAAAAGTCTTTGTAAGAAAAGATGTCAAAAAGTAGGAAAGTTGT
AATAAAAATAGAAAATCAAAAATCAAAAAGTAACTTTAGGGAAATTTAAATGTTTTAATT
TATTAATTCAACATAAGTAAATACTATACAATAGTGGAATTAAGAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11