Sequences from confirmation amplicon


Confirmation sequences for line 554C10 and hit At4g33900 are shown.
Line ID 554C10
Locus At4g33900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16252581
Primers used for PCR 02kk - 8474, show primer details
   
Sequence Name 47-K113531-0022-554-C10C-02kk
GenBank Accession LN506253 [GenBank]
Sequencing primer gene specific primer, 02kk: 5'-TGACCAAGCCTCTTTATTGTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113531-0022-554-C10C-02kk
AAGATGTGGGCTTTACGTGTTTGGCTTTAACTTCTTTTTAGAATTAGGGTCTTTGTTTCT
GATTCTTATTTAAAGAATCCTTACGTTGTAGAATAGATTAGTTTTGGATTATTGAATAAA
GAGTTTTAAGAGAGCTTTCTATAAAGCCTAGAGAGGAGGACTCTCAACCCTAAGGGCTTG
CAAAACCAAGTCGATATCACTACCGATCTCGACTTTATCTCTATTGCTTCTTCATCAAGT
TTTGACGTTCTAAGCAAGATCCGTTTGTAAATCCGAGTTCTCCTAGAGATCTTGTGTTCT
CGTCCGATATTTAGCTTCCGCTCTCTATCAACAACCGTAAGAGAAAAAAAGTGATCAACA
ACCATGGAATATGGAGAAGAACCCTCGATTAAAAGATTTCTAATGCTTCCTGATGATTTG
GTATTTAACTGCTTAGCTCGCGTCTCAAGATTACATTATCCAACTCTCTCATTAGTCTCC
AAAAAATTTCGCTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 22-K113530-0022-554-C10C-8409
GenBank Accession LN496195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113530-0022-554-C10C-8409
TTGAAGCGAAATTTTTTGGAGACTAATGAGAGAGTTGGATAATGTAATCTTGAGACGCGA
GCTAAGCAGTTAAATACCAAATCATCAGGAAGCATTAGAAATCTTTTAATCGAGGGTTCT
TCTCCATATTCCATGGTTGTTGATCACTTTTTTTCTCTTACGGTTGTTGATAGAGAGCGG
AAGCTAAATATCGGACGAGAACACAAGATCTCTAGGAGAACTCGGATTTACAAACGGATC
TTGCTTAGAACGTCAAAACTTGATGAAGAAGCAATAGAGATAAAGTCGAGATCGGTAGTG
ATATCGACTTGGTTTTGCAAGCCCTTAGGGTTGAGAGTCCTCCTCTCTAGGCTTTATAGA
AAGCTCTCTTAAAACTCTTTATTCAATAATCCAAAACTAATCTATTCTACAACGTAAGGA
TTCTTTAAATAAGAATCAGAAACAAAGACCCTAATTCTAAAAAGAAGTTAAAAGCCAAAC
ACGTAAAGCCCACATCTTTAATAAAGTCCATAAACTACTAAATCATGAATGAGAAACAAT
AAAGAGCCTTTGGTC

Last Updated on 06.12.2018 16:11