Sequences from confirmation amplicon


Confirmation sequences for line 554E06 and hit At5g52390 are shown.
Line ID 554E06
Locus At5g52390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21264590
Primers used for PCR JL46 - 8474, show primer details
   
Sequence Name 24-K101383-0022-554-E06C-8409
GenBank Accession LN496929 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K101383-0022-554-E06C-8409
CATTTACATATCGATCTCTACTTCAACCTTGCTGCTGGTGAAGGCTACGATATTGGTAGA
CCAGTACGGTTATATTACTTTTCTCTCTCTAATAGATTATGGTATAATTTATTTGTTTGG
TTTGCTATAGGAGTGTATTTACCAAAGCTATGTGAATCACAAGAAGGGAAGTCAAGAAGA
GCAATGTCGGAAATTAGGAGCTCGGGAATTGCAATGGACACTCTTGCACCGGTTGGACCA
GTCATGTTGGGCGAGATAGCACCTGAGCCGGCTACTTCAATGGACAACATGCCTTACGTG
CCGGCACCTTCACCGTATTAATTAAGGCAAGGGAAAATGGAGAGGACACGTATGATATCA
TGAGTTTTCGACGAGAATAATTAAGAGATTTATGTTTAGTTCGACGGTNTTAGTATTACA
TCGTTTATTGCGTCCTTATATATATGTACTTCATAAAAACACACCACGACACATTAAGAG
ATGGTGAAAGTAGGCTGCGTTCTGGTGTAACTTTT
   
Sequence Name 24-K101389-0022-554-E06C-JL46
GenBank Accession LN496930 [GenBank]
Sequencing primer gene specific primer, JL46: 5'-TGTAAAAGTTACACCAGAACGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K101389-0022-554-E06C-JL46
GTGTTTTTATGAAGTAATATATATAAGGACGCAATAAACGCTTTAATACTAAAACCGTCG
AACTAAACATAAATCTCTTAATTATTCTCGTCGAAAACTCATGATATCATACGTGTCCTC
TCCATTTTCCCTTGCCTTAATTAATACGGTGAAGGTGCCGGCACGTAAGGCATGTTGTCC
ATTGAAGTAGCCGGCTCAGGTGCTATCTCGCCCAACATGACTGGTCCAACCGGTGCAAGA
GTGTCCATTGCAATTCCCGAGCTCCTAATTTCCGACATTGCTCTTCTTGACTTCCCTTCT
TGTGATTCACATAGCTTTGGTAAATACACTCCTATAGCAAACCAAACAAATAAATTATAC
CATAATCTATTAGAGAGAGAAAAGTAATATAACCGTACTGGTCTACCAATATCGTACCTT
CACCAGCAGCAAGGTTGAAGTAGAGATCGATATGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAGAAAGAGTAATTACCAAT
TTTTTTTTCATTTCAAAATGTAGATGTCCCGCACGTTA

Last Updated on 06.12.2018 16:11