Sequences from confirmation amplicon


Confirmation sequences for line 554G10 and hit At5g48360 are shown.
Line ID 554G10
Locus At5g48360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19596583
Primers used for PCR 32fm - 8474, show primer details
   
Sequence Name 60-K111411-0022-554-G10C-32fm
GenBank Accession LN510294 [GenBank]
Sequencing primer gene specific primer, 32fm: 5'-GAATCTGACCATATTAGAACCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111411-0022-554-G10C-32fm
GACCATTACCACCATTACCTCCTCGTAGCTTCCACCATAACAACTATGAAACAGAGGTAA
ACGAAGAAGACGAAGAAGAAGAAGAAGATGTGTTCTTCTCACCAATGGCTTCTCTCCCTG
GCTCAGCGAACTCAAGCCCTTCTCATTCATGCTCTTCCTCCTGCTCCGGTTGGGTTTCTC
CAGCTAGGTCATTCTCTATCACCATGTCTCCACCAAACCCTAGATACTCAGACGCCACTA
ACCTCCAGTCTCCGTCACCGGAGAGACTAAGAGTAAGGAAGATACGAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
ACGTTATT
   
Sequence Name 54-K111410-0022-554-G10C-8409
GenBank Accession LN508517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K111410-0022-554-G10C-8409
TTCATTGAATTATCCTCGTATCTTCCTTACTCTTAGTCTCTCCGGTGACGGAGACTGGAG
GTTAGTGGCGTCTGAGTATCTAGGGTTTGGTGGAGACATGGTGATAGAGAATGACCTAGC
TGGAGAAACCCAACCGGAGCAGGAGGAAGAGCATGAATGAGAAGGGCTTGAGTTCGCTGA
GCCAGGGAGAGAAGCCATTGGTGAGAAGAACACATCTTCTTCTTCTTCTTCGTCTTCTTC
GTTTACCTCTGTTTCATAGTTGTTATGGTGGAAGCTACGAGGAGGTAATGGTGGTAATGG
TCGTATCTCCGGCGAGTCTTGCTTCAAGAAAAAAGTACCTCCGGTTCTAATATGGTCAGA
TT

Last Updated on 06.12.2018 16:11