Sequences from confirmation amplicon


Confirmation sequences for line 556B08 and hit At4g22560 are shown.
Line ID 556B08
Locus At4g22560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11880065
Primers used for PCR AB68 - 8474, show primer details
   
Sequence Name 24-K105918-0022-556-B08C-AB68
GenBank Accession LN496993 [GenBank]
Sequencing primer gene specific primer, AB68: 5'-CCGCTATCACTCTACAAATCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105918-0022-556-B08C-AB68
CTCAGTACGCTTCCCACTACCCGAAAACGTACACACAGCCTGACCTTTCCCACCGGCAAA
AACACACGCGCCACCGTTAAACCCATCAAGAACCGGATAAAACCTCATCATCTCATTACC
ATCAGCCAAACACCGTGCATCCTCTTCTTCACACGTGCCTCCGTTAAAACCCGCACGTGA
CTTCACAACCTCTCTATACTCCTCGAACCGGGTTACAGTCCTTGACCCGTGTTCCACTTT
GAAAATCATCTCGATCCGACCCGGAAACTCGTCGGAGCTCCAGCTTGAATGAAATATAAT
CTCCACTACGTTTCTTGACGGGTGACCATCGGGCAGCTCGGTCAGTGCGTCTGATGTTCG
GGCAGATTTGCTTCGTCGGGTCCGGTTTGGCGGGTTAAGTAACTCTGTTTCGGTTCGACA
AGACTTGAAACGCCTTAGATTCGGGTCGGGTTTCTGGTTTTTCTTGCTTAACCGGGTATC
GATCACGTCTTTGACGCANTGTGAGCTCTTTCGACTACAATTGTAACTCGACGACGACNT
TAGTGATTTACTGTTGAAATTGTAGACATCATCGAACGCTTTTGATTTGCATTGCAATGA
TTTGATCCACCCATTTGGCATGTTCGGGTTCTTGGATCCGGATCTTTATTGTTCTTGTCT
CTCTTTCTCTCTTCAACAAGTTATGTATATGTATGTTATGAACAAAAAAAATACCCGGAT
ACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGA
   
Sequence Name 24-K105919-0022-556-B08C-8409
GenBank Accession LN496994 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105919-0022-556-B08C-8409
TTACATTGAATATATCCTGGTATCCGGGTATTTTTTTTGTTCATAACATACATATACATA
ACTTGTTGAAGAGAGAAAGAGAGACAAGAACAATAAAGATCCGGATCCAAGAACCCGAAC
ATGCCAAATGGGTGGATCAAATCATTGCAATGCAAATCAAAAGCGTTCGATGATGTCTAC
AATTTCAACAGTAAATCACTAATGTCGTCGTCGAGTTACAATTGTAGTCGAAAGAGCTCA
CATTGCGTCAAAGACGTGATCGATACCCGGTTAAGCAAGAAAAACCAGAAACCCGACCCG
AATCTAAGGCGTTTCAAGTCTTGTCGAACCGAAACAGAGTTACTTAACCCGCCAAACCGG
ACCCGACGAAGCAAATCTGCCCGAACATCAGACGCACTGACCGAGCTGCCCGATGGTCAC
CCGTCAAGAAACGTAGTGGAGATTATATTTCATTCAAGCTGGAGCTCCGACGAGTTTCCG
GGTCGGATCGAGATGATTTTCAAAGTGGAACACGGGTCAAGGACTGTAACCCGGTTCGAG
GAGTATAGAGAGGTTGTGAAGTCACGTGCGGGTTTTAACGGAGGCACGTGTGAAGAAGAG
GATGCACGGTGTTTGGCTGATGGTAATGAGATGATGAGGTTTTATCCGGTTCTTGATGGG
TTTAACGGTGGCGCGTGTGTTTTTGCCGGTGGGAAAGGTCAGGCTGTGTGTACGTTTTCG
GGTAGTGGTGAAGCGTACGTGAGTAGTGGAGGTGGCGGAGGAAGGAAGGCGATGATGATT
TGTA
Primers used for PCR 63wn - 8474, show primer details
   
Sequence Name 58-K118471-0022-556-B08C-63wn
GenBank Accession LN509831 [GenBank]
Sequencing primer gene specific primer, 63wn: 5'-ATATGCTTCATTCCGATGTTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118471-0022-556-B08C-63wn
TTTTTTTCCTTTTTTCTCTACCTTTTCTTTTGACAATAATGGGGGTGTTTTTTTAGTTTG
TATGTTCAAATAAAACTTAAGAATCGGTCCTACTGATTCCCTTACCTTATTGTTACACTA
CAGTAAAGTTACTCGGATTCCTATTTATGATTGTCAATATCTCAATAAATAAAACCCGAC
ACAACACTTAAATCATTCAATTCTTGATATGTTAAAAGGAAACACTGCGTCAATTTGCTT
TCCTCATTAAATGTAAAGAAAATTCTTGCTTTCTTCACCTAACTTCTCTCTTATCACTCT
GCCTGTTTTAATCATAAATTCATTCTTCAATAACTTTAGTTTCAAGAAAGAATAAATAAA
AATCCTTATCAACAAAATCAGTCGCTTGAATACACACACACACAAAAAAAAAAATCAAAT
ACAAATAATAAGTTTGGATTTCATAAAATATTTGTTTAAAAACTTATAATCAATCTTTTA
TACAATTTGAGTCACCACATCAAACCAAATAAGTATCATATTTTTCTAGGTTTTGATTAT
GTAAACCATATGGGCCAGGCCCAGGAAAAAAATTAGAGGGTAAAATCGTAATATGAATGA
TCAATTGTAAATGGCTTCATGTCCGGAAAATCTACTTGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAA
   
Sequence Name 26-K118470-0022-556-B08C-oriL
GenBank Accession LN498135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118470-0022-556-B08C-oriL
TTTACATTGATCATTCATATTACGATTTTACCCTCTAATTTTTCTCCTGGGCCTGGCCCA
TATGGTTTACATAATCAATACCTAGAAAAATATGATACTTATTTGGTTTGATGTGGTGAC
TCAAATTGTATAATAGATTGATTATAAGTTTCTAAACAAATATTTTATGAAATCCAAACT
TATTATTCGTATCTGATTTTTTTTTTTGTGTGTGTGTGTATTCAAGCGACTGATTTTGTT
GATAAGGATTTTTATTTATTCTTTCTTGAAACTAAAGTTATTGAAAAATGAATTTATGAT
TAAAACAGGCAAAGTGATAAAAGAAAATTTAGGTGAAAAAAGCAAAAATTTTCTTTACTT
TTAATGAGGAAAGCAAATTGACCCAGTGTTTCCTTTTAACATATCAAAAATTGAATGATT
TAAGTGTTGTGTCGGGTTTTATTTATTGAGATATTGACAATCATAAATAGGAATCCAAGT
AACTTTACTGTAGTGTAACAATAAGGTAAGGGAATCAGTAGGACCGATTCTTAATTTTTA
TTTGAACATACAAACTAAAAAAACCCCCCCTTTATTGTCAAAAGAAAAGGTAGAGAAAAA
GGAAAAAAACTTGTATTAAAATGTCATATTGTACACTTTTACAAGTATGAAAAACA
Primers used for PCR AR04 - 8474, show primer details
   
Sequence Name 57-K118471-0022-556-B08C-AR04
GenBank Accession LN509548 [GenBank]
Sequencing primer gene specific primer, AR04: 5'-AATCAGTCGCTTGAATACACACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118471-0022-556-B08C-AR04
CTAAAATTTTTGTTTAGAAACTTATAATCAATCTATTATACAATTTGAGTCACCACATCA
AACCAAATAAGTATCATATTTTTCTAGGTATTGATTATGTAAACCATATGGGCCAGGCCC
AGGAAAAAAATTAGAGGGTAAAATCGTAATATGAATGATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAGAG
TAATTACCATTTTTTTTTCATTTCAAAAATGTAAATTTCC
   
Sequence Name 25-K118470-0022-556-B08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118470-0022-556-B08C-8409
ATTGAAGACCCTATGCTGAACGAAGCATCTTCAATATGTCATCCCAGACTTGCCCAAGGG
GTCCGGTCCTGCACATCCACTGGGATAACCACTGAGTATGATGGTCAATATGGAGAAACT
GAATGAGTAATTACCGATTTTTTTTCAATTC

Last Updated on 06.12.2018 16:11