Sequences from confirmation amplicon


Confirmation sequences for line 556H01 and hit At4g23210 are shown.
Line ID 556H01
Locus At4g23210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12150369
Primers used for PCR 47wx - 8474, show primer details
   
Sequence Name 57-K119006-0022-556-H01C-47wx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47wx: 5'-CTGCAACTATCGCCTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K119006-0022-556-H01C-47wx
TACATCTCGTACGATTACTGCAGCCTCCGCAGCAAGAAGCACACCATCGTCCAGTGATAA
CCATTACAGAGTTGATTTTGCGAACTTGACAAAATTCCAGAATATCTACGCATTGATGCA
ATGCACGCCAGATATTTCTTCTGACGAATGTAACAACTGTCTCCAACGAGGCGTACTTGA
GTACCAGTCATGCTGTGGAAATAATACTGGAGGCTATGTTATGCGGCCAATCTGCTTTTT
CCGGTGGCAGCTTTTTACATTCTCTAAGGCTTTTCATAACATTACCTTGGCTACTACTCC
TCCTCTTTCTCCTCCTCCACTGCAACGTCCTGTGGTTGCGTCACAACCACCTTCTGCAGA
CAACCGGGACAAGAAGAGAGACAATAGTAAGTCCTTGCAAACTTATGACTTAAAGTTACA
GTCACAAGACTCTGTCCAAAGACTTATAAGGTTTTTTTTTTCATTCTACAGGTAGCGGAA
AAATCTCAATGAAAACTATTCTAGCAATTGTTGTGGTTGGTATAGTCATCCTCATCATCA
TCTCGGGAATTCTTGCTCGCCGGTTTGCTCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAG
   
Sequence Name 27-K119005-0022-556-H01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K119005-0022-556-H01C-8409
ACGGCGAGCAGAATTCCCGAGATGATGATGAGGATGACTATACCAACCACAACAATTGCT
AGAATAGTTTTCATTGAGATTTTTCCGCTACCTGTAGAATGAAAAAAAAAACCTTATAAG
TCTTTGGACAGAGTCTTGTGACTGTAACTTTAAGTCATAAGTTTGCAAGGACTTACTATT
GTCTCTCTTCTTGTCCCGGTTGTCTGCAAAAGGTGGTTGTGACGCAACCACAGGACGTTG
CAGTGGAGGAGGAGAAAGAGGAGGAGTAGTAGCCAAGGTAATGTTATGAAAAGCCTTAAA
GAATGTAAAAAGCTGCCACCGGAAAAAGCAAATTGGCCGCATAACATAGCCTCCAGTATT
ATTTCCACAGCATGACTGGTACTCAAGTACGCCTCGTTGGAGACAGTTGTTACATTCGTC
AAAAGAAATATCTGGCGTGCATTGCATCAATGCGTAGATATTCTGGAATTTTGTCAAGTT
CGCAAAATCAACTCTGTAATGGTTATCACTGGACGATGGTGTGCTTCTTGCTGCGGAGGC
TGCAGTAATCGTACAAGATGTTAAGTCTTCCCATATTGTCTTGAACTCTGTTAGATTTGA
GGCGATAGTTGCAG

Last Updated on 06.12.2018 16:11