Sequences from confirmation amplicon


Confirmation sequences for line 558G02 and hit At1g53650 are shown.
Line ID 558G02
Locus At1g53650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20031247
Primers used for PCR KF07 - 8474, show primer details
   
Sequence Name 45-K103355-0022-558-G02C-KF07
GenBank Accession LN505497 [GenBank]
Sequencing primer gene specific primer, KF07: 5'-CCATATGAAAGACCACACCCTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103355-0022-558-G02C-KF07
AATATAACATTTTACAATTATATTAAATAATAATATTAGTAATGTGTAATATATTTCTTA
GCAAAAAAAGTGTACTAAAACTACTAAAGATTTTTTTCAATATAAAAAATGTAATAAACT
GGTGTATGTGTAAAATATGTAATTATCTAAAGGCCCTCAAGAGGAACATAAAATAATAAA
GAATCATAACTTTTCTTTTCTTTTTAATTTTACATTTGACGAAATTAATTAAGAAAAAAA
TCTTAGTCCGAAAAAAAAGAGAAGCACTCTTGTTAGAGAGTTTTGGTCTATTTGATTGAG
CTAGAAAAAGGCCTAAGCAACAAACCAAAACCCATCATTCCTTCTTCTTCTTCTTCTTCT
CATCCATCGCCACCTTCTGAAACCACCGCACTAGATTTATATATTGTCTACGATTAAGCA
TTCTCGTATTCTGAAATGGGTGTTCAATAAAACTCGATTAATCTCATCAAATTTTGCATC
TTTTTTACGAAATTCGACTGAAGAGAGAAGGAAAAGTTCGCAACTTTTTCTTTCCTCTGT
CTCTNGTTTTTTGTTGAATTGTGATCCTTTTTGTTGTTTTATATTTTTTTGGAATTTTCT
GATCTAATTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCC
   
Sequence Name 61-K103356-0022-558-G02C-8409
GenBank Accession LN510494 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103356-0022-558-G02C-8409
TTAGATCAGAAAATTCAAAAAAATATAAAACAACAAAAAGGATCACAATTCAACAAAAAA
CAAGAGACAGAGGAAAGAAAAAGTTGCGAACTTTTCCTTCTCTCTTCAGTCGAATTTCGT
AAAAAAGATGCAAAATTTGATGAGATTAATCGAGTTTTATTGAACACCCATTTCAGAATA
CGAGAATGCTTAATCGTAGACAATATATAAATCTAGTGCGGTGGTTTCAGAAGGTGGCGA
TGGATGAGAAGAAGAAGAAGAAGAAGGAATGATGGGTTTTGGTTTGTTGCTTAGGCCTTT
TTCTAGCTCAATCAAATAGACCAAAACTCTCTAACAAGAGTGCTTCTCTTTTTTTTCGGA
CTAAGATTTTTTTCTTAATTAATTTCGTCAAATGTAAAATTAAAAAGAAAAGAAAAGTTA
TGATTCTTTATTATTTTATGTTCCTCTTGAGGGCCTTTAGATAATTACATATTTTACACA
TACACCAGTTTATTACATTTTTTATATTGAAAAAAATCTTTAGTAGTTTTAGTACACTTT
TTTTGCTAAGAAATATATTACACATTACTAATATTATTATTTAATATAATTGTTAAAATG
TTATTATTTTTGTTATGGATATTAGATAGGGTGTGGCTTTCCATATGG

Last Updated on 06.12.2018 16:11