Sequences from confirmation amplicon


Confirmation sequences for line 560F05 and hit At2g41130 are shown.
Line ID 560F05
Locus At2g41130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17144105
Primers used for PCR RU70 - 8474, show primer details
   
Sequence Name 27-K027064-022-560-F05C-8409
GenBank Accession LN498232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K027064-022-560-F05C-8409
CCATGTAGATTTCCCGGACATGAAGCCATGTAGATCACTTTTTGGGAAAGTTCTCAAGCT
TTTTCAGTTGATTTGATTTGATACAAACATCAAATTTCAACTTCTTGATCCAAACTTTTC
ATTGTAATTTCATGTTTCTATTTTCAGACCGATAAAGCCACACTGCTCGCCAAAGTAGTT
CAACGAGTCAGAGAACTTAAACAGCAAACCCTAGAGACCTCCGACTCCGACCAAACATTA
TTACCATCAGAGACCGACGAAATTAGTGTTCTACACTTTGGAGACTATTCAAACGACGGT
CATATAATCTTCAAAGCCTCTCTATGTTGTGAAGATAGATCAGATCTCTTGCCGGACCTT
ATGGAGATTCTCAAGTCTCTTAACATGAAGACTCTCCGAGCTGAGATGGTAACCATTGGT
GGTCGGACAAGAAGTGTTCTTGTCGTAGCTGCTGACAAAGAGATGCACGGCGTCGAGTCT
GTGCATTTTTTGCAAAATGCTCTCAAGTCGCTGCTTGAGCGGTCAAGCAAGTCGTTGATG
GAACGTAGTTCTGGGGGTGGAGGAGGAGAACGGTCAAAGCGGCGTCGTGCGCTGGATCAC
ATCATAATGGTGTGA
   
Sequence Name 28-K027064-022-560-F05C-RU70
GenBank Accession LN498697 [GenBank]
Sequencing primer gene specific primer, RU70: 5'-TCAATTCTCATCATTTCACACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K027064-022-560-F05C-RU70
ATGATGTGATCCACCGCACGACGCCGCTTTGACCGTTCTCCTCCTCCACCACCAGAACTA
CGTTCCATCAACGACTTGCTTGACCGCTCAAGCAGCGACTTGAGAGCATTTTGCAAAAAA
TGCACAGACTCGACGCCGTGCATCTCTTTGTCAGCAGCTACGACAAGAACACTTCTTGTC
CGACCACCAATGGTTACCATCTCAGCTCGGAGAGTCTTCATGTTAAGAGACTTGAGAATC
TCCATAAGGTCCGGCAAGAGATCTGATCTATCTTCACAACATAGAGAGGCTTTGAAGATT
ATATGACCGTCGTTTGAATAGTCTCCAAAGTGTAGAACACTAATTTCGTCGGTCTCTGAT
GGTAATAATGTTTGGTCGGAGTCGGAGGTCTCTAGGGTTTGCTGTTTAAGTTCTCTGACT
CGTTGAACTACTTTGGCGAGCAGTGTGGCTTTATCGGTCTGAAAATAGAAACATGAAATT
ACAATGAAAAGTTTGGATCAAGAAGTTGAAATTTGATGTTTGTATCAAATCAAATCAACT
GAAAAAGCTTGAGAACTTTCCCAAAAAGTGATCTACATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACCATTTT
TGATAANAACGACAAATTACGATCCGTCGTATTTATGGCGCAAGCAATAAACAAATTATT
CTAATTC

Last Updated on 06.12.2018 16:11