Genotyping and primer details


Genotyping and primer details for 560F05 - At2g41130
Line ID 560F05
Locus At2g41130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17144105 of line 560F05 Chr2:17144105
   
Insertion allele amplicon 8474 - RU70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RU70: 5'-TCAATTCTCATCATTTCACACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17144701
   
wt amplicon (over InsPos) 22rd - RU70
wt amplicon start Chr2:17143790
wt amplicon end Chr2:17144701
wt amplicon size 912 bp
Locus specific primer RU70: 5'-TCAATTCTCATCATTTCACACCAT-3'
2nd locus specific primer 22rd: 5'-TGCATCTTCTCTAACCCAGAGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11