Sequences from confirmation amplicon


Confirmation sequences for line 562B05 and hit At3g48560 are shown.
Line ID 562B05
Locus At3g48560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18003001
Primers used for PCR MK31 - 8474, show primer details
   
Sequence Name 21-K031809-022-562-B05C-8409
GenBank Accession LN495553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031809-022-562-B05C-8409
CATTTCAAAATGCGTTGTTAGATAGTGTTCCTCTTGTAGCAATCACAGGACAAGTCCCTC
GTCGTATGATTGGTACAGATGCGTTTCAAGAGACTCCGATTGTTGAGGTAACGCGTTCGA
TTACGAAGCATAACTATCTTGTGATGGATGTTGAAGATATCCCTAGGATTATTGAGGAAG
CTTTCTTTTTAGCTACTTCTGGTAGACCTGGACCTGTTTTGGTTGATGTTCCTAAAGATA
TTCAACAACAGCTTGCGATTCCTAATTGGGAACAGGCTATGAGATTACCTGGTTATATGT
CTAGGATGCCTAAACCTCCGGAAGATTCTCATTTGGAGCAGATTGTTAGGTTGATTTCTG
AGTCTAAGAAGCCTGTGTTGTATGTTGGTGGTGGTTGTTTGAATTCTAGCGATGAATTGG
GTAGGTTTGTTGAGCTTACGGGGATCCCTGTTGCGAGTACATTGATGGGGCTGGGATCTT
ATCCTTGTGATGATGAGTTGTCGTTGCATATCCTTGGAATGCATGGTACTGTGTATGC
   
Sequence Name 22-K031809-022-562-B05C-MK31
GenBank Accession LN495999 [GenBank]
Sequencing primer gene specific primer, MK31: 5'-ACAGCGTAATTTGCATACACAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031809-022-562-B05C-MK31
ACGACACTCATCATCACAAGGATAAGATCCCAGCCCCATCAACGTACTCGCAACAGGGAT
CCCCGTAAGCTCAACAAACCTACCCAATTCATCGCTAGAATTCAAACAACCACCACCAAC
ATACAACACAGGCTTCTTAGACTCAGAAATCAACCTAACAATCTGCTCCAAATGAGAATC
TTCCGGAGGTTTAGGCATCCTAGACATATAACCAGGTAATCTCATAGCCTGTTCCCAATT
AGGAATCGCAAGCTGTTGTTGAATATCTTTAGGAACATCAACCAAAACAGGTCCAGGTCT
ACCAGAAGTAGCTAAAAAGAAAGCTTCCTCAATAATCCTAGGGATATCTTCAACATCCAT
CACAAGATAGTTATGCTTCGTAATCGAACGCGTTACCTCAACAATCGGAGTCTCTTGAAA
CGCATCTGTACCAATCATACGACGAGGGACTTGTCCTGTGATTGCTACAAGAGGAACACT
ATCTAACAACGCATTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11