Sequences from confirmation amplicon


Confirmation sequences for line 562E05 and hit At5g16410 are shown.
Line ID 562E05
Locus At5g16410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5365779
Primers used for PCR 17xo - 8474, show primer details
   
Sequence Name 30-K119356-0022-562-E05C-17xo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17xo: 5'-GAACTGAAATGTGGGAGCGTAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K119356-0022-562-E05C-17xo
TTATTCCTGAACTGTTCCTCGTCTCGTGGGCCGAGATATCTCGATCCGATATACCGATTT
CGTATGTACCTTTGCTCAAAAGATCCCTCTTAAAGCCACGACGACCCTTGATCATCGACT
CTTCAATAGACAAACTGTACATGCCTATAACGTCCTTGACCGTTCCACAAGAAATCACTA
ATCAAGATAATATCCTAACAAGCCGTATCTATTACATCAAAGCAGATGTCTTAGAAAAGT
TTCAAACTCTGGCTACTAATGGTAAGAGGACCAAACTCGAGTCTTTCAGTGCGTTTCTAT
GGAAACTCTTGGCTAAACACGCTGCAACAGAGTCGGTACTCCCGACTAAAACCTCGAAAC
TTGGCATCGTTGTTGATGGAAGGAAGAAACTTATGGAGCAAGAGAACTGCAATTACTTTG
GGAACGTCCTCTCAGTTCCATTTGGAGAGCGGAGAATCGATGACTTGATCCACAAGCCAT
TGTCTTGGGTGACAGACGAGGTTCACAAGTTACTGGAGAGTACAATGACCAAAGACCATT
TCTTGAACTTGATCGATTGGGTTGAGACTAGCCGTCCGATACCAGTTATATCAAGGATCT
ACAGTACAGGATCCAACGATGGACCCGCTTTCGTTGTTTCGTCTGGAAAGAGTTTTCCAG
TGGCCCGGATTGATTTCGGGTGGGGCTCGCCTGTTTTTGGATCGTACCATCTCCCACCCG
GGAGCAGAGCTGGTTACGTAATGACAATGCCGAGTCCGGTGGAAAACGGTGGCTCCGGAG
ATTGGACGGTTTACTTGCATCTTACGGGAAATAGGGAAATCTACATGGATCAGCAATGAG
TATGATGGGTCATATGGAGAAAAAGAAAGAGTAATTACCAA
   
Sequence Name 10-K119355-0022-562-E05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119355-0022-562-E05C-8409
ACCGTCCAATCTCCGGAGCCACCGTTTTCCACCGGACTCGGCATTGTCATTACGTAACCA
GCTCTGCTCCCGGGTGGGAGATGGTACGATCCAAAAACAGGCGAGCCCCACCCGAAATCA
ATCCGGGCCACTGGAAAACTCTTTCCAGACGAAACAACGAAAGCGGGTCCATCGTTGGAT
CCTGTACTGTAGATCCTTGATATAACTGGTATCGGACGGCTAGTCTCAACCCAATCGATC
AAGTTCAAGAAATGGTCTTTGGTCATTGTACTCTCCAGTAACTTGTGAACCTCGTCTGTC
ACCCAAGACAATGGCTTGTGGATCAAGTCATCGATTCTCCGCTCTCCAAATGGAACTGAG
AGGACGTTCCCAAAGTAATTGCAGTTCTCTTGCTCCATAAGTTTCTTCCTTCCATCAACA
ACGATGCCAAGTTTCGAGGTTTTAGTCGGGAGTACCGACTCTGTTGCAGCGTGTTTAGCC
AAGAGTTTCCATAGAAACGCACTGAAAGACTCGAGTTTGGTCCTCTTACCATTAGTAGCC
AGAGTTTGAAACTTTTCTAAGACATCTGCTTTGATGTAATAGATACGGCTTGTTAGGATA
TTATCTTGATTAGTGATTTCTTGTGGAACGGTCAAGGACGTTATAGGCATGTACAGTTTG
TCTATTGAAGAGTCGATGATCAAGGGTCGTCGTGGCTTTAAGAGGGATCTTTTGAGCAAA
GGTACATACGAAATCGGTATATCGGATCGAGATATCTCGGCCCACGAGACGAGGAACATG
TTCATGGAATAAGCATCTGCTATACGGTGATCAAATGTGCATCCCACTACTACGCTC

Last Updated on 06.12.2018 16:11