Sequences from confirmation amplicon


Confirmation sequences for line 562G04 and hit At4g01610 are shown.
Line ID 562G04
Locus At4g01610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:696805
Primers used for PCR KS39 - 8474, show primer details
   
Sequence Name 61-K033096-022-562-G04C-8409
GenBank Accession LN510438 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K033096-022-562-G04C-8409
CCATTTACATTGAATATATCCTGCACATTTCATACATGTCAACAACATTCATATGAAAAC
AAAACTAAATCTAGAACTATTCGTAATTTAGTACTAATAAAAACTTACATCACCCCAGCC
TCTGTTCCATTGATTTGCCATCAACTAAACACAAGAGAAAATACAAACCAAGAACGTTAT
AAATTTCGGTTAATTTCGAATTCTCGATAAAAAAATCTCACGAAAATGTATAACATTACC
CAATAATCTTCTCCTTCACTGCTAGTTCCCCAACCAATAAGTTTAACAGCATGACCTCCA
ATGTTAGAACCTGTTATGTGCTTATAGACTCCAGATTTGTAATGAGCAAAATCCTAACCA
AAGCAAACCATTAATGATACATCTTTCTTTAACTTCTATTCAAAAACAATCTTAGGATTT
AAGTTTTATTACCTCATAAACGGTAAAAGAGACCCAAAACAGGT
   
Sequence Name 13-K033096-022-562-G04C-KS39
GenBank Accession LN491932 [GenBank]
Sequencing primer gene specific primer, KS39: 5'-GACCTGTTGAAGTCTCTTTTACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K033096-022-562-G04C-KS39
TATCCTAGATTGTTTTTGATAGAAGTTAAAGAAAGATGTATCATTAATGGTTTGCTTTGG
TTAGGATTTTGCTCATTACAAATCTGGAGTCTATAAGCACATAACAGGTTCTAACATTGG
AGGTCATGCTGTTAAACTTATTGGTTGGGGAACTAGCAGTGAAGGAGAAGATTATTGGGT
AATGTTATACATTTTCGTGAGATTTTTTTATCGAGAATTCGAAATTAACCGAAATTTATA
ACGTTCTTGGTTTGTATTTTCTCTTGTGTTTAGTTGATGGCAAATCAATGGAACAGAGGC
TGGGGTGATGTAAGTTTTTATTAGTACTAAATTACGAATAGTTCTAGATTTAGTTTTGTT
TTCATATGAATGTTGTTGACATGTATGAAATTTGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11