Sequences from confirmation amplicon


Confirmation sequences for line 563A08 and hit At1g12130 are shown.
Line ID 563A08
Locus At1g12130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4120011
Primers used for PCR 12sn - 8474, show primer details
   
Sequence Name 26-K116390-0022-563-A08C-12sn
GenBank Accession LN498082 [GenBank]
Sequencing primer gene specific primer, 12sn: 5'-TTCACATAACCCTTTGTTTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116390-0022-563-A08C-12sn
TATGGCATCCGCAATATCAGTTTCCCGTCTTGGAAAACAACCGAACCATCCTCATACACT
CGGTCAATCTATAACATATCAGGGTGGTTGGGGAGGAAGAAATCAAGTAATCATATATTA
AAATTAAATGGTGTTTTTGTTATTTTCTCTGTGTGGTCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAATA
   
Sequence Name 01-K116389-0022-563-A08C-8409
GenBank Accession LN486729 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116389-0022-563-A08C-8409
ACCATTTAATTTTATATATGATTACTTGATTTCTTCCTCCCCACCACCCTGATATGTTAT
AGATTGACCGAGTGTATGAGGATGGTTCGGTTGTTTTCCAAGACGGGAAACTGATATTTG
CGGATGCCATAGTACATTGCACCGGCTACAAGTATTGCTTCCCATTTCTTGAAACAAAGG
GTTATGTGAAA

Last Updated on 06.12.2018 16:11