Sequences from confirmation amplicon


Confirmation sequences for line 563F02 and hit At1g49100 are shown.
Line ID 563F02
Locus At1g49100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18167329
Primers used for PCR EU02 - 8474, show primer details
   
Sequence Name 01-K103353-0022-563-F02C-EU02
GenBank Accession LN486536 [GenBank]
Sequencing primer gene specific primer, EU02: 5'-GTTGAAGGAAACCCTAAGCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103353-0022-563-F02C-EU02
GATACCTGGAGAGGTGGACATCCCAAAAGAGTATAATTGTACCGGTTGTCTCATCAGTTG
CTTTAATAGCTATTCTTATAGCTGCATTGGTTTTGTTTTTGGTTCTTAGAAAGAAAAATC
CATCAAGGAGTAAAGGTATTTATGGTGAACGCATAAGCTCTTATAATTACTATACACTAG
TAGAAACTATACAAAATCTATGAAATTTTTATATTGCAGAAAATGGTAGAACTTCAAGAT
CATCCGAGCCACCAAGAATAACAAAAAAGAAAAAGTTTACTTACGTGGAAGTTACTGAAA
TGACAAATAACTTTAGAAGTGTTCTTGGGAAAGGAGGGTTCGGTATGGTTTATCATGGAT
ATGTAAATGGTAGAGAGCAAGTTGCTGTTAAAGTACTCTCACACGCTTCAAAACATGGCC
ATAAACAATTCAAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
   
Sequence Name 17-K103354-0022-563-F02C-8409
GenBank Accession LN493813 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103354-0022-563-F02C-8409
TTTCATTGAATATATCCTGCTTTGAATTGTTTATGGCCATGTTTTGAAGCGTGTGAGAGT
ACTTTAACAGCAACTTGCTCTCTACCATTTACATATCCATGATAAACCATACCGAACCCT
CCTTTCCCAAGAACACTTCTAAAGTTATTTGTCATTTCAGTAACTTCCACGTAAGTAAAC
TTTTTCTTTTTTGTTATTCTTGGTGGCTCGGATGATCTTGAAGTTCTACCATTTTCTGCA
ATATAAAAATTTCATAGATTTTGTATAGTTTCTACTAGTGTATAGTAATTATAAGAGCTT
ATGCGTTCACCATAAATACCTTTACTCCTTGATGGATTTTTCTTTCTAAGAACCAAAAAC
AAAACCAATGCAGCTATAAGAATAGCTATTAAAGCAACTGATGAGACAACCGGTACAATT
ATACTCTTTTTGGGATGTCCACCTTCTCCAGGTTTATTTCCACATGGTCCTTTTGTGCAA
AGAAGCTTAGGGTTTCCTTCAA
Primers used for PCR NK68 - 3144, show primer details
   
Sequence Name 43-K106973-0022-563-F02C-3144
GenBank Accession LN504850 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K106973-0022-563-F02C-3144
TTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTATAACACCAAAATATTCCTCTATGCAAG
TGTCTTTTGTTAGTTTTCAATAAATATGGACCATAGATTTGAAATATATGTGTGCTTTTG
TGGTTCATTAGGTTGAACTTCTTTTGAGAGTTCATCACAAGAATTTGGTAAGCCTAGTTG
GATACTGCGAAAAAGGGAAGGAATTGGCTCTTGTCTACGAATACATGGCTAATGGAGACT
TAAAAGAGTTTTTCTCAGGTAAACCAAATTTAAGTATTATACCAGACACCTAATCTTAAT
CCATTTTCGATGGAACAAAAACATGTAACAAATCATTCTTGGTCATAAGTTAACGTCCTG
GTTAAAAAACACCCAGGGAAGCGTGGTGATGATGTTTTAAGGTGGGAAACTAGATTACAA
ATAGCAGTGGAGGCCGCACAAGGTTAGTAAAGCCTTATATATGAACCTATTATTCTCTTA
AAACTTTAGTTTTGTATGACATATATAGGTCCAAATGAATTTGTGACACTAGGTTTGGAG
TACTTGCATAAAGGATGTAA
   
Sequence Name 59-K106974-0022-563-F02C-NK68
GenBank Accession LN510013 [GenBank]
Sequencing primer gene specific primer, NK68: 5'-GAACAATTGGTGGTCTACATCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K106974-0022-563-F02C-NK68
AATTCATTTGGACCATATATGTCATACAAAACTAAAGTTTTAAGAGAATAATAGGTTCAT
ATATAAGGCTTTACTAACCTTGTGCGGCCTCCACTGCTATTTGTAATCTAGTTTCCCACC
TTAAAACATCATCACCACGCTTCCCTGGGTGTTTTTTAACCAGGACGTTAACTTATGACC
AAGAATGATTTGTTACATGTTTTTGTTCCATCGAAAATGGATTAAGATTAGGTGTCTGGT
ATAATACTTAAATTTGGTTTACCTGAGAAAAACTCTTTTAAGTCTCCATTAGCCATGTAT
TCGTAGACAAGAGCCAATTCCTTCCCTTTTTCGCAGTATCCAACTAGGCTTACCAAATTC
TTGTGATGAACTCTCAAAAGAAGTTCAACCTAATGAACCACAAAAGCACACATATATTTC
AAATCTATGGTCCATATTTATTGAAAACTAACAAAAGACACTTGCATAGAGGAATATTTT
GGTGTTATAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCA
AATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGT
CATCCCTTACGTCAGTGGAGATATC

Last Updated on 06.12.2018 16:11