Sequences from confirmation amplicon


Confirmation sequences for line 564B12 and hit At3g25160 are shown.
Line ID 564B12
Locus At3g25160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9161480
Primers used for PCR CH29 - 8474, show primer details
   
Sequence Name 25-K105918-0022-564-B12C-CH29
GenBank Accession LN497445 [GenBank]
Sequencing primer gene specific primer, CH29: 5'-AATTCACCGTTAGGAATCACAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105918-0022-564-B12C-CH29
GCCGCCGGAATTTGATCCTCATCTACAAACTCACTCGCCAAAAGACTTGCTCCGGTAACA
ATAAAAATCCCACCACCTTATCATAATTCATAAGATTTTGACTTTAAATCATAGACCTTT
GTTTCTTGGTAATGTGATTAGAAGTGAAAACATTTATATGTTTATATTGTGACAATGTTG
TGGAATCTTATAAGCTCTTTTGGTTTTGCTAGTGAACATTTATGTTTATATTGTATCAAT
GTTGTGTGGAACTTTATAAGCTCATTTTGGTTTTGTCTCATAGAATATGTGTATGGTTTG
AATTATTAGGTCTTTCTTTAAAATCTCAAGAAGTGACAGCAATATTTCTAGCGGTGAGAT
TGATATGCATCTACAAAGGCTAGACAACATCCCCTGAAACCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGATTTA
   
Sequence Name 25-K105919-0022-564-B12C-8409
GenBank Accession LN497446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105919-0022-564-B12C-8409
CCTTTNAGATGCATATCAATCTCACCGCTAGAAATATTGCTGTCACTTCTTGAGATTTTA
AAGAAAGACCTAATAATTCAAACCATACACATATTCTATGAGACAAAACCAAAATGAGCT
TATAAAGTTCCACACAACATTGATACAATATAAACATAAATGTTCACTAGCAAAACCAAA
AGAGCTTATAAGATTCCACAACATTGTCACAATATAAACATATAAATGTTTTCACTTCTA
ATCACATTACCAAGAAACAAAGGTCTATGATTTAAAGTCAAAATCTTATGAATTATGATA
AGGTGGTGGGATTTTTATTGTTACCGGAGCAAGTCTTTTGGCGAGTGAGTTTGTAGATGA
GGATCAAGATTCCGGCGGCGTGGATGAGCTCGGAGGCGATGAAAAAGTGATTGTGATTCC
AACGGGTGAAT

Last Updated on 06.12.2018 16:11