Sequences from confirmation amplicon


Confirmation sequences for line 565C02 and hit At1g09760 are shown.
Line ID 565C02
Locus At1g09760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3159176
Primers used for PCR KQ55 - 8474, show primer details
   
Sequence Name 02-K101713-0022-565-C02C-8409
GenBank Accession LN486972 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101713-0022-565-C02C-8409
GAGCCTTTACATTGCTTATGCAATTGAATATATTCGGTTTTGATCCATATTGGCATGTAG
ATTTCCCGGACATGTAGATTTCTTTTCAATATCATCAATTACCGAGAAACTAATTTAGAA
AAGAAATCACATCGCTGAAGCAAGAGATGAAACTGCAGGCCATGTTATGCGTAGTGAAGA
TAGCCGTTCATACAAAGTATATGTAGAAGGTCAAGCGCCATTAAGCACCTCTTCAAATCA
AAACGTACGAAGAACGTAAAATAAAAAGAGAGGGAGAGAGAGATCATTCATATGTCCTCT
CTTAGTTGCAGGGCTCAGGAGCAATTCCTATGCGAGAATTAGGAACGTCAAACAAGATCC
TTAAGTTCTGCTGTTGGAGATTCGCGATCACGTTTAGAACAGCGTTTGCGTTCTGCCGTA
TCCCGGCCATGGATAGACACGTCAGCGTTCCCGCGCTGCTGTGGATAAGCGTATTCTCCA
TCGGCAGCTTCAAGTCGAGTGACGTCATGTGCAGCGTTATCTTTGGCGCCACATTCTCGT
TATCCGCTGAGAAACACGTGTCAAACGCTCCCAATGTTGAGAATGACGACACATTCACCT
TCATTAACAAGATTGTAATTAGTTTCCCATTTCTTGAGTTATACTAATTGTGATATTAAC
GAAATTACCTGCTTCC
   
Sequence Name 02-K101719-0022-565-C02C-KQ55
GenBank Accession LN486973 [GenBank]
Sequencing primer gene specific primer, KQ55: 5'-TTAGAGACGAGTTTAGGAAGCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101719-0022-565-C02C-KQ55
TTAGTATAACTCAGAATGGGAAACTAATTACAATCTTGTTAATGAAGGTGAATGTGTCGT
CATTCTCAACATTGGGAGCGTTTGACACGTGTTTCTCAGCGGATAACGAGAATGTGGCGC
CAAAGATAACGCTGCACATGACGTCACTCGACTTGAAGCTGCCGATGGAGAATACGCTTA
TCCACAGCAGCGCGGGAACGCTGACGTGTCTATCCATGGCCGGGATACGGCAGAACGCAA
ACGCTGTTCTAAACGTGATCGCGAATCTCCAACAGCAGAACTTAAGGATCTTGTTTGACG
TTCCTAATTCTCGCATAGGAATTGCTCCTGAGCCCTGCAACTAAGAGAGGACATATGAAT
GATCTCTCTCTCCCTCTCTTTTTATTTTACGTTCTTCGTACGTTTTGATTTGAAGAGGTG
CTTAATGGCGCTTGACCTTCTACATATACTTTGTATGAACGGCTATCTTCACTACGCATA
ACATGGCCTGCAGTTTCATCTCTTGCTTCAGCGATGTGATTTCTTTTCTAAATTAGTTTC
TCGGTAATTGATGATATTGAAAAGAAATCTACATGTCCGGGAAATCTACATGCCAATATG
GATCAAAACCGAATATATTCAATTGCATAAGCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCANAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11