Genotyping and primer details


Genotyping and primer details for 565C02 - At1g09760
Line ID 565C02
Locus At1g09760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3159176 of line 565C02 Chr1:3159176
   
Insertion allele amplicon 8474 - KQ55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KQ55: 5'-TTAGAGACGAGTTTAGGAAGCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3158571
   
wt amplicon (over InsPos) 09dn - KQ55
wt amplicon start Chr1:3158571
wt amplicon end Chr1:3159550
wt amplicon size 980 bp
Locus specific primer KQ55: 5'-TTAGAGACGAGTTTAGGAAGCAGG-3'
2nd locus specific primer 09dn: 5'-CCTTGTTCAAGAAATCTGGATAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11