Sequences from confirmation amplicon


Confirmation sequences for line 565F11 and hit At2g35605 are shown.
Line ID 565F11
Locus At2g35605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14946230
Primers used for PCR 30fp - 8474, show primer details
   
Sequence Name 27-K111325-0022-565-F11C-30fp
GenBank Accession LN498447 [GenBank]
Sequencing primer gene specific primer, 30fp: 5'-GATGACATGATAGACCGATGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K111325-0022-565-F11C-30fp
GTTTTGTTTATCAAATAAATCCAAAAAGGCCCAGAATCTTGGTGCAATAGGAGGCCCAAA
ACAGGCCCAAACTAAGAAGAGCTAAACCACAGACCTTCATTAATTTTTAAAGGGCTGAGA
GAAAGAGAGAGACTGGGAAACGTTTCAGTTTTCAAAGTCTGTGAGTTTGATCTAGGGTTT
TAGACAGAGCTCTCTCTCCTTCCAATTTCAAAACTGTGTGAAGAAGAAAAAATGTCGAGG
GTTTTCGGAGCTTGCAGGGTACTGATGGCGAAGGCCGCTTCTTCCACCGGCGCTAAAACT
GGTAGACAAGGTACTGGCATTCTTAAGGTGGTTCCCGTTTCGAAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 11-K111324-0022-565-F11C-8409
GenBank Accession LN491174 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K111324-0022-565-F11C-8409
CAGTTTTAGCGCCGGTGGAAGAAGCGGCCTTCGCCATCAGTACCCTGCAAGCTCCGAAAA
CCCTCGACATTTTTTCTTCTTCACACAGTTTTGAAATTGGAAGGAGAGAGAGCTCTGTCT
AAAACCCTAGATCAAACTCACAGACTTTGAAAACTGAAACGTTTCCCAGTCTCTCTCTTT
CTCTCAGCCCTTTAAAAATTAATGAAGGTCTGTGGTTTAGCTCTTCTTAGTTTGGGCCTG
TTTTGGGCCTCCTATTGCACCAAGATTCTGGGCCTTTTTGGATTTATTTGATAAACAAAA
CAACAATTCGAAGCCTATAAATGTCAAAAGAAAGCAATGGCATTCATCGGTCTTCNATGT
CAT

Last Updated on 06.12.2018 16:11