Genotyping and primer details


Genotyping and primer details for 565F11 - At2g35605
Line ID 565F11
Locus At2g35605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14946230 of line 565F11 Chr2:14946230
   
Insertion allele amplicon 8474 - 30fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30fp: 5'-GATGACATGATAGACCGATGAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14945867
   
wt amplicon (over InsPos) 11gf - 30fp
wt amplicon start Chr2:14945867
wt amplicon end Chr2:14946730
wt amplicon size 864 bp
Locus specific primer 30fp: 5'-GATGACATGATAGACCGATGAATG-3'
2nd locus specific primer 11gf: 5'-TTAGGCTTTGAAATGTTGACTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11