Sequences from confirmation amplicon


Confirmation sequences for line 566B03 and hit At2g27310 are shown.
Line ID 566B03
Locus At2g27310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11684441
Primers used for PCR LB24 - 8474, show primer details
   
Sequence Name 59-K033718-022-566-B03C-8409
GenBank Accession LN509915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K033718-022-566-B03C-8409
CATTAGTTTCTGCTCTCTTCCATATCTTTCACCCCGCTTCGTAGTCCCCACCCGGATATC
GAATCCGGGTTTGGACCGACTCTTTCGGGTCGAGTATATCGACCCGAAACGGTGATGACA
AGAACCATCCTGATTTACCTTTCTCTGTTTCCATCTCTTGTACCTTCGAGTATATAATCT
CTCCTCTGTAGTATAAATCAACGGCTGAGATTAATCCCGTCGGTGGATCATGTTTTTCAG
ACTGCCACGTGTGTTCAGTGAACGGGTACGAGTCAGCAAAGAAAGAACGGTAACCGGAAG
GGAAAGAGGAGATAGCTTGGACAACACGTGGATCATTGATCGAAGGCCACGTGTCGATGG
AGAGTTCTTGCCAGAGTTTCTCTTCCGTGCATAGAGTTTGGAGGTAAGAAGAGGTTGTTG
CGGTGGAGGCTAGCGTCGGACCATCGAGTCGGGTCAAGATTTGGGTTTGGANTATGTCA
   
Sequence Name 21-K033718-022-566-B03C-LB24
GenBank Accession LN495572 [GenBank]
Sequencing primer gene specific primer, LB24: 5'-CTGACATAATCCAAACCCAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K033718-022-566-B03C-LB24
TCGAGCTGCCTCACCGCAACACCTCTTCTTACCTCCAACTCTATGCACGGAAGAGAAACT
CTGGCAAGAACTCTCCATCGACACGTGGCCTTCGATCAATGATCCACGTGTTGTCCAAGC
TATCTCCTCTTTCCCTTCCGGTTACCGTTCTTTCTTTGCTGACTCGTACCCGTTCACTGA
ACACACGTGGCAGTCTGAAAAACATGATCCACCGACGGGATTAATCTCAGCCGTTGATTT
ATACTACAGAGGAGAGATTATATACTCGAAGGTACAAGAGATGGAAACAGAGAAAGGTAA
ATCAGGATGGTTCTTGTCATCACCGTTTCGGGTCGATATACTCGACCCGAAAGAGTCGGT
CCAAACCCGGATTCGATATCCGGGTGGGGACTACGAAGCGTGGGTGAAAGATATGGAAGA
GAGCATGAAACTTAATTGGATACTAATCGATCCGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTC

Last Updated on 06.12.2018 16:11