Sequences from confirmation amplicon


Confirmation sequences for line 567A09 and hit At5g16980 are shown.
Line ID 567A09
Locus At5g16980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5579710
Primers used for PCR NC57 - 8474, show primer details
   
Sequence Name 43-K101219-0022-567-A09C-8409
GenBank Accession LN504772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101219-0022-567-A09C-8409
TTTCGCAATTGATACTCATTTACTTATTTCTAAGTGTTTTATGCCTATAAATGTTAGAAT
CCGTTTGCATAATAAAGCGGCAATACTTTTTGATTGATCTTCTCAATTGCGTTTTGACCA
ATCTAAGAGATATTCTCCGTTTTTTTCCAGGTGTTTCCCTAATGGCATTGACATATACTT
TGAGAATGTAGGAGGCAAAATGCTAGATGCAGTGCTTATGAACATGAACATGCACGGGCG
TATCGCTGTCTGTGGAATGATCTCACAGTACAATCTTGAGAACCAAGAAGGTGTACACAA
CCTATCCAACATAATCTACAAACGAATCCGCATTCAAGGCTTTGTAGTGTCTGATTTCTA
CGACGAATACTCAAAGTTCTTGGAGTTTCTGCATCCGCACATTAAAGAAGGGAAGATAAC
GTACGTGGAAGATGTAGCTGATGGGCTCGAGAAAGGTCCTGAAGCTCTCGTGGGACTCTT
CCATGGTAAGAATGTTGGGAAACAA
   
Sequence Name 43-K101226-0022-567-A09C-NC57
GenBank Accession LN504773 [GenBank]
Sequencing primer gene specific primer, NC57: 5'-CTTGTTTCCCAACATTCTTACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101226-0022-567-A09C-NC57
TTTCTGAGCCCTCANCTACATCTTCACGTACGTTATCTTCCCTTCTTTAATGTGCGGATG
CAGAAACTCCAAGAACTTTGAGTATTCGTCGTAGAAATCAGACACTACAAAGCCTTGAAT
GCGGATTCGTTTGTAGATTATGTTGGATAGGTTGTGTACACCTTCTTGGTTCTCAAGATT
GTACTGTGAGATCATTCCACAGACAGCGATACGCCCGTGCATGTTCATGTTCATAAGCAC
TGCATCTAGCATTTTGCCTCCTACATTCTCAAAGTATATGTCAATGCCATTAGGGAAACA
CCTGGAAAAAAACGGAGAATATCTCTTAGATTGGTCAAAACGCANTTGAGAAGATCAATC
AAAAAGTATTGCCGCTTTATTATGCAAACGGATTCTAACATTTATAGGCATAAAACACTT
AGAAATAAGTAAATGAGTATCAATTGCGAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11