Sequences from confirmation amplicon


Confirmation sequences for line 567C12 and hit At5g11660 are shown.
Line ID 567C12
Locus At5g11660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3749367
Primers used for PCR 65ye - 8474, show primer details
   
Sequence Name 39-K119974-0022-567-C12C-65ye
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 65ye: 5'-CATATCTTACATCGGTTACCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K119974-0022-567-C12C-65ye
CTTTCTCATATATAGTAAAAAGACCTACATGTCAAATTTGATTTACCAACATAACATATT
TGCTACTAAAATGTAAAGTATGTATATTACCTGTAACATGCTAGCATAAACAGCTCTTTC
TTGTCCTTTGTGTGGATATGGAAAAGGTGCACTTAAAAGATCAGTTTTTGACACTTGAAC
AAAACCCGGACATATTGTATTATAACAACCAGTTCCATTGGCACCCTGCCAATATTTAAT
CTTAATATATTAATATATTCCATATGATAATTGAAAAAATAGTAAAATGTAATGTACATT
AAAAGATAGAAGCGTAAAACACATACTAAGTGTCAAATTATTTCCCTATAGATAAATTAT
TTTGCAATCAAAAGGGTATATATTTAGTATTTACAAAAGAAAATTCCGAGAATATTTAGC
ATTTCATGGGAAACGAGATAAAGAAGTATTTGTATCTAGATGTACCTTCCAAACCCCAAA
ACTCCGTGTACGTCCATCACCAAATAAACTTGGATTCACCTGTAAATCATAGTAATGTAA
CCTATTTTTAAAACCAATATATATA
   
Sequence Name 07-K119973-0022-567-C12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K119973-0022-567-C12C-8409
ATTTATTACTTGAGTTACAAATGTGTATATTTGGAAAATATGTATGATATATTTTTTTTG
GAAATTTGAAGTATGCTGGAGTTAAATCACAAGGTGATGGTCCTTATCATGGGTTGGCAG
CTTATATGAGTGTGCATGATTTGAATATTAGTAGGGACCAAACTTCATATGCTAGCATTT
TTGTTGGTAGTGGAGACAACAAGAAAATTAATTTTATTCAAACCGGTTGGATGGTAAATT
TTATGTTGATGTTATTAAATTTATATATATATATATATATTTATATATATTGGTTTTAAA
AATAGGTTACTTTACTCTGACTTAGAGG

Last Updated on 06.12.2018 16:11