Sequences from confirmation amplicon


Confirmation sequences for line 567F06 and hit At4g11030 are shown.
Line ID 567F06
Locus At4g11030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6738371
Primers used for PCR TH17 - 8474, show primer details
   
Sequence Name 54-K103582-0022-567-F06C-TH17
GenBank Accession LN508450 [GenBank]
Sequencing primer gene specific primer, TH17: 5'-TTGCAATTAATATAGAAGGCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103582-0022-567-F06C-TH17
GACAAAACCATCACGTAAGAGCAAGAAAAGAAGCTAAAAAAGGATCTTACAAGAATTGAC
ACCAAACAATTCAGTATTCATTCATTTTGAGCCAAAGATATAACTTAGTAATCATTCTTA
CGTACAAAACATGAAATAAAATTCCCAAGATCCCTGATCGATATACGTTTATCATATTAA
CATGTTTCAAAATGATAATGCATGCTACACTCTAAATCAAAACGTATATGAGCACACAAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 70-K103583-0022-567-F06C-8409
GenBank Accession LN512372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103583-0022-567-F06C-8409
ATATACGTTTTGATTTAGAGTGTAGCATGCATTATCATTTTGAAACATGTTAATATGATA
AACGTATATCGATCAGGGATCTTGGGAATTTTATTTCATGTTTTGTACGTAAGAATGATT
ACTAAGTTATATCTTTGGCTCAAAATGAATGAATACTGAATTGTTTGGTGTCAATTCTTG
TAAGATCCATTTTTAGCTTCTTTTCTTGCTCTTACGTGATGGTTTTGTCTGACATGGTAC
ATGGCATTATTTAAATAATTTTGGGGTGGCCTTCTTATNAAATTGCA

Last Updated on 06.12.2018 16:11