Sequences from confirmation amplicon


Confirmation sequences for line 570A06 and hit At1g52600 are shown.
Line ID 570A06
Locus At1g52600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19591327
Primers used for PCR 52gp - 8474, show primer details
   
Sequence Name 47-K112250-0022-570-A06C-52gp
GenBank Accession LN506236 [GenBank]
Sequencing primer gene specific primer, 52gp: 5'-TGAGACAAAGAGAAATGGGAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K112250-0022-570-A06C-52gp
AAACATTTCACCTGCTCGATCGGGTCTTTACTCATGTGCAAGAACAATATATCCCCCTAT
AAGATATCACATTCAATACAATTTTGAGTTAACAAACAGAAATATGCTGACCCTCAAATA
GATTATTAGTAGCTGATTCATCCACAAAGAGAGAGTAAAGGCGCTGTAAAATCTTATGTA
TCCCGAAAACCTAAGTCCAATTACCCTCTTAAAACCGGGTTCCATGCTTCCAGAGAGAAC
AACCACCACCGGAGATTCGCTTCCAGTGACACACATCAAAGCTTTCCATATGATCAAAGC
AGATGTGACAATCATCCCTACCAGAATTCAACACAGAGAGCAATTTATCATTCACTTAAG
ACACAACTTCTGTCATATAACTCATATTGAAAACAAATAATTCAATATTCGATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 37-K112249-0022-570-A06C-8409
GenBank Accession LN502664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112249-0022-570-A06C-8409
CATTTCATCGAATATTGAATTATTTGTTTTCAATATGAGTTATATGACAGAAGTTGTGTC
TTAAGTGAATGATAAATTGCTCTCTGTGTTGAATTCTGGTAGGGATGATTGTCACATCTG
CTTTGATCATATGGAAAGCTTTGATGTGTGTCACTGGAAGCGAATCTCCGGTGGTGGTTG
TTCTCTCTGGAAGCATGGAACCCGGTTTTAAGAGGGTAATTGGACTTAGGTTTTCGGGAT
ACATAAGATTTTACAGCGCCTTTACTCTCTCTTTGTGGATGAATCAGCTACTAATAATCT
ATTTGAGGGTCAGCATATTTCTGTTTGTTAACTCAAAATTGTATTGAATGTGATATCTTA
TAGGGGGATATATTGTTCTTGCACATGAGTAAAGACCCGATTCGAGCAGGTGAAATTGTT
GTTTTCAATGTTGATGTAAGTTTCCCATTTTTTTTTGTCTC

Last Updated on 06.12.2018 16:11