Sequences from confirmation amplicon


Confirmation sequences for line 570B04 and hit At1g48540 are shown.
Line ID 570B04
Locus At1g48540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17949421
Primers used for PCR 31uj - 8474, show primer details
   
Sequence Name 45-K117776-0022-570-B04C-31uj
GenBank Accession LN505672 [GenBank]
Sequencing primer gene specific primer, 31uj: 5'-GAGATAAAAATGAAGGGGACATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117776-0022-570-B04C-31uj
ATAACTCTGGATTGCTGACAATTTGAGGGAACCCATTTTTCATTAATCTAGGATAACATA
GGCAAAGAGAGAATATACCTCCACGAGTGGTGCTCTGAAAATGGAGAACATTGTACTGGA
ATATACTTAGCTTCAAACCTCCACATATTTCTTTCTCAAACAAGTCCACCTGAATATTCT
CCAGACTGCCATTAACATAGAAGACAAAAATTATGACAGCAAAAATATACAGAGCCGACT
CTTGGTTGAATGTGAATGGCTCACCTTTGCAAAGAACATTTATACTCTGTAGCCTGGGAA
TCGAAAACTTGTGTGATATTGAGTAATTCTGTTGGCATTTACAATTGGACAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGGTTATT
   
Sequence Name 21-K117775-0022-570-B04C-8409
GenBank Accession LN495841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117775-0022-570-B04C-8409
TTGAATATATCCTGTCCAATTGTAAATGCCAACAGAATTACTCAATATCACACAAGTTTT
CGATTCCCAGGCTACAGAGTATAAATGTTCTTTGCAAAGGTGAGCCATTCACATTCAACC
AAGAGTCGGCTCTGTATATTTTTGCTGTCATAATTTTTGTCTTCTATGTTAATGGCAGTC
TGGAGAATATTCAGGTGGACTTGTTTGAGAAAGAAATATGTGGAGGTTTGAAGCTAAGTA
TATTCCAGTACAATGTTCTCCATTTTCAGAGCACCACTCGTGGAGGTATATTCTCTCTTT
GCCTATGTTATCCTAGATTAATGAAAAATGGGTTCCCTCAAATTGTCAGCAATCCAGAGT
TATAGGTTTCAATGTTTCATATCCACATTAAGTCATGTCCCCTTCTTTTTTATCTC

Last Updated on 06.12.2018 16:11