Sequences from confirmation amplicon


Confirmation sequences for line 570E03 and hit At2g20840 are shown.
Line ID 570E03
Locus At2g20840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8971939
Primers used for PCR 13vy - 8474, show primer details
   
Sequence Name 04-K118356-0022-570-E03C-13vy
GenBank Accession LN488112 [GenBank]
Sequencing primer gene specific primer, 13vy: 5'-ACGTATTGAGTGGGAATATCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118356-0022-570-E03C-13vy
GATACTTAACAGTTGTGGACTTTGAGTAAGGCTAACTTTTGGTGAGGTGGTGGCAGATAT
TTTACTTCATTGGGTTTGGGTTCTTCTGTCTGGAGTCACTCGTCAGCATCTGGGTTATTC
AGGTTCCCTCCTTTGCTGAATCATTTATTCATGTAGATAAGTTAAAAAGGAAGAAGTGGT
TAACAAGTTGTTGTTGTTGTTGTGTGTGTGTGTGGGAACAGCAAGTGTACATGTACTTCA
GGGGGAGCGGGAAAGCAGCAGAGATGAAGCAGGAGGCCACAAGACGAGCAATGAAGCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTATT
   
Sequence Name 04-K118355-0022-570-E03C-8409
GenBank Accession LN488111 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118355-0022-570-E03C-8409
ATTGAATATATCCTGCTTCATTGCTCGTCTTGTGGCCTCCTGCTTCATCTCTGCTGCTTT
CCCGCTCCCCNCTGAAGTACATGTACACTTGCTGTTCCCACACACACACACAACAACAAC
AACAACTTGTTAACCACTTCTTCCTTTTTAACTTATCTACATGAATAAATGATTCAGCAA
AGGAGGGAACCTGAATAACCCAGATGCTGACGAGTGACTCCAGACAGAAGAACCCAAACC
CAATGAAGTAAAATATCTGCCACCACCTCACCAAAAGTTAGCCTTACTCAAAGTCCACAA
CTGTTTAAGTTATTCGCTACCTAAGCTTTCAACTTACCCCGACCAAGATATTCCCACTCA
ATACAT

Last Updated on 06.12.2018 16:11