Sequences from confirmation amplicon


Confirmation sequences for line 570E08 and hit At3g03940 are shown.
Line ID 570E08
Locus At3g03940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1014623
Primers used for PCR PB29 - 8474, show primer details
   
Sequence Name 57-K102504-0022-570-E08C-8409
GenBank Accession LN509377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K102504-0022-570-E08C-8409
TTTACAATTGAATATATCCTGTTCTACAAGTATTGAGCAGCATCATACTCATTCATCGTT
TTAAAATTGGGTTTCATCGGTTATTTTTCGTTTAGGTATACTCAATATAAGAGCTGCTTG
ATCAGCTGTTGCAGCCATTGATGTTATCCTGTATCCACTTTCCCACCTTCGATGTATTCC
TTCACTTGGGTACAAGAAGTCAAGCTCCACCACCTATAATATACACCACATAAACCTCGT
TAATACATTATAGTGCTCAAAAAGGAAACAAAGAAAAGTTGAAATGCCTCAGGTAAAGAG
ATCGGAACCTGTTCAGAGTAGCCTGAGTTCCTGGACATTACTACACCCCAACGACTCCCA
GCGGTGGTCATGGATGTTACATGAAAACCTTCTTTCCACTTTTTATTTATCCACTTGAAT
GGAAATGAGTCGCTGACTTTGTAAGATTGCTGGGTATAAGGAGTTCCTATCGCATTACAT
GAAGTGGAATAAGTAAAACGGCTTCAATGCTTT
   
Sequence Name 57-K102505-0022-570-E08C-PB29
GenBank Accession LN509378 [GenBank]
Sequencing primer gene specific primer, PB29: 5'-TAAAGCATGAAAGCCGTTTTACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K102505-0022-570-E08C-PB29
GACTCCTTATACCCGCAATCTTACAAGTCAGCGACTCATTTCCATTCAAGTGGATAAATA
AAAAGTGGAAAGAAGGTTTTCATGTAACATCCATGACCACCGCTGGGAGTCGTTGGGGTG
TAGTAATGTCCAGGAACTCAGGCTACTCTGAACAGGTTCCGATCTCTTTACCTGAGGCAT
TTCAACTTTTCTTTGTTTCCTTTTTGAGCACTATAATGTATTAACGAGGTTTATGTGGTG
TATATTATAGGTGGTGGAGCTTGACTTCTTGTACCCAAGTGAAGGAATACATCGAAGGTG
GGAAAGTGGATACAGGATAACATCAATGGCTGCAACAGCTGATCAAGCAGCTCTTATATT
GAGTATACCTAAACGAAAAATAACCGATGAAACCCAATTTTAAAACGATGAATGAGTATG
ATGCTGCTCAATACTTGTAGAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11