Sequences from confirmation amplicon


Confirmation sequences for line 571C09 and hit At5g04380 are shown.
Line ID 571C09
Locus At5g04380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1235242
Primers used for PCR JD09 - 8474, show primer details
   
Sequence Name 53-K027356-022-571-C09C-8409
GenBank Accession LN508032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K027356-022-571-C09C-8409
CATTTACAATTGAAGACAACCTAAATCGGCTACCTTAATGCATTTACGAAAGTCCAAGTT
TGTCAACATTTCCTCTGTGTTTTTAACTAAGATCGGATTGGTTATTGATAAAACTCTTTT
CTGAAAACATAGATAAAACACATGTTAAGAAATGATACTTTAATTTGTGTATGCATGTCT
TATAGAAACATATACTATATGTAGAGAAGAAGAAAGAACCTGAAGAAGAGACTTGGTGGT
GTAACTGTTGTCTACATCTCCACCATTCATACATAGAGCACCCACAAAAGGATATTTACT
ACTTCTCTCTTCATCCACTTTATACTCACTTTTACAATCACAACCTGC
   
Sequence Name 54-K027356-022-571-C09C-JD09
GenBank Accession LN508357 [GenBank]
Sequencing primer gene specific primer, JD09: 5'-ATGCAGGTGTGATTGTAAAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K027356-022-571-C09C-JD09
GTAATATCCTTTTGTGGGTGCTCTATGTATGAATGGTGGAGATGTAGACAACAGTTACAC
CACCAAGTCTCTTCTTCAGGTTCTTTCTTCTTCTCTACATATAGTATATGTTTCTATAAG
ACATGCATACACAAATTAAAGTATCATTTCTTAACATGTGTTTTATCTATGTTTTCAGAA
AAGAGTTTTATCAATAACCAATCCGATCTTAGTTAAAAACACAGAGGAAATGTTGACAAA
CTTGGACTTTCCTAAATGCATTAAGGTAGCCGATTTAGGTTGTTCTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11