Sequences from confirmation amplicon


Confirmation sequences for line 572C12 and hit At1g80000 are shown.
Line ID 572C12
Locus At1g80000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30096248
Primers used for PCR 28dl - 8474, show primer details
   
Sequence Name 50-K110039-0022-572-C12C-28dl
GenBank Accession LN507200 [GenBank]
Sequencing primer gene specific primer, 28dl: 5'-CCACCTTATACTGTCCTTGATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K110039-0022-572-C12C-28dl
TACCTTCCTCTGCCGGATCGTCCAGGTTTAGAAATAATTTTCTCAGTATTGTTAATCAAC
ATGTCATTTCCATATGCATATGTGTAGACTCTTTGCTTTTTACCTTAGAGGAAGAGTAGT
ATGGGAATGCAGCTTTAAGCCCTTAACTAAGTAACGTATGTGAGATTTTAGATTTGTGGT
TCCTCTTATTCTAGACCCACTGCAAAATGCTTGTTACCTGTTAGATTTACATTTCTTAAG
TGCTTAGATTAGTAGTTTCTCGACTATGTACACCCAGAAACATGTAGTTTTCATTAAACA
GACTCACTTTCTTGGTTGGGCACTCATGTAACCATGTCAATCATCTCTGATTATGAGCGT
CTAGTAAAGCATATAATTTGATATTTGGGTAAGAGTACCGGCCTCGCAAATCGGCGAAAA
CGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGATCTTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 34-K110038-0022-572-C12C-8409
GenBank Accession LN501454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K110038-0022-572-C12C-8409
TTACACTTGATATATCCTGAAGATCCACATCATCTGTGGGAAACTCGCGTAAAATCAGGC
GTTTTCGCCGATTTGCGAGGCCGGTACTCTTACCCAAATATCAAATTATATGCTTTACTA
GACGCTCATAATCAGAGATGATTGACATGGTTACATGAGTGCCCAACCAAGAAAGTGAGT
CTGTTTAATGAAAACTACATGTTTCTGGGTGTACATAGTCGAGAAACTACTAATCTAAGC
ACTTAAGAAATGTAAATCTAACAGGTAACAAGCATTTTGCAGTGGGTCTAGAATAAGAGG
AACCACAAATCTAAAATCTCACATACGTTACTTAGTTAAGGGCTTAAAGCTGCATTCCCA
TACTACTCTTCCTCTAAGGTAAAAAGCAAAGAGTCTACACATATGCATATGGAAATGACA
TGTTGATTAACAATACTGAGAAAATTATTTCTAAACCTGGACGATCCGGCAGAGGAAGGT
AACCCTGGTTTGTCTGCTTGGTAAGAACCATCAAGGACAGATTAAGGTGG

Last Updated on 06.12.2018 16:11