Sequences from confirmation amplicon


Confirmation sequences for line 574C02 and hit At5g59650 are shown.
Line ID 574C02
Locus At5g59650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24031447
Primers used for PCR 43iv - 8474, show primer details
   
Sequence Name 83-K113077-0022-574-C02C-43iv
GenBank Accession LN514332 [GenBank]
Sequencing primer gene specific primer, 43iv: 5'-TAAATGTGAATCCTCTGCTTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K113077-0022-574-C02C-43iv
AATGTGTATAATTAGTGATAAATTATCCGATATGATTCAATCAAAGTCACTGACCACTAG
AAAGTCAAATCTCTTGCTTTCTCTCTGTGTGAACATTTTTTAATGCGTTAAAAGAGTGGT
GACTGTAAAACCCTCTATTGACATATTCTCTTAAAAACACAGAAGTTGCATATTATTTTT
TCTAAATACAAGTTGCATATATTCTGACATTCTTGGGACAAATCAAATTTGTAATTGTAA
AGTTGCAATAGAAAACACATTTACTCATTCGTTTCACTGACTCAGGACATCTTGCCATGT
TGCCAGAGGCTTGGACTGTTTCTTGGGTTTATCTATTTATACGCTGTGTTTCTCTCAAAC
TCTGGCATTGGTAAGGTTTTGATATCTAAAAGCAATTATGGATAGTCCTTGTTGGCTTTT
GCTGCTGCTATTAGGAGCTTTTGCCATTATTGGTTGTGTTCAAGCTCAGGATCAACAAGG
TACCTAAATCTTAATGGTTATGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 56-K113075-0022-574-C02C-8409
GenBank Accession LN509157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113075-0022-574-C02C-8409
TTTCATTGAATATATACATAACCATTAAGATTTAGGTACCTTGTTGATCCTGAGCTTGAA
CACAACCAATAATGGCAAAAGCTCCTAATAGCAGCAGCAAAAGCCAACAAGGACTATCCA
TAATTGCTTTTAGATATCAAAACCTTACCAATGCCAGAGTTTGAGAGAAACACAGCGTAT
AAATAGATAAACCCAAGAAACAGTCCAAGCCTCTGGCAACATGGCAAGATGTCCTGAGTC
AGTGAAACGAATGAGTAAATGTGTTTTCTATTGCAACTTTACAATTACAAATTTGATTTG
TCCCAAGAATGTCAGAATATATGCAACTTGTATTTAGAAAAAATAATATGCAACTTCTGT
GTTTTTAAGAGAATATGTCAATAGAGGGTTTTACAGTCACCACTCTTTTAACGCATTAAA
AAATGTTCACACAGAGAGAAAGCAAGAGATTTGACTTTCTAGTGGTCAGTGACTTTGATT
GAATCATATCGGATAATTTATCACTAATTAATACACATTTTGACGTCTTCATCGACAAGA
ACAGCTTCTAATCACAAAGCA

Last Updated on 06.12.2018 16:11