Sequences from confirmation amplicon


Confirmation sequences for line 575G07 and hit At3g58570 are shown.
Line ID 575G07
Locus At3g58570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21659138
Primers used for PCR 49yl - 8474, show primer details
   
Sequence Name 28-K120167-0022-575-G07C-49yl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 49yl: 5'-TGGGAGTAAGAGGGAACTAAGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K120167-0022-575-G07C-49yl
ACTGAAGAAAGAGTGAGATTTGGTAGGTCTGGGAAATGAGAAGGATGATCGGTACATAAT
CTTGTTTTCCTTTATTACTGGAGGGTTCAATTCGTTGTCTAAATAATGTTTATTGTTTAA
GTTGACACTACCAAAATTTTGGCTCTTAAAATTACAAGATAGAAGTTGCGTACTGTAATT
ACCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAT
AGATATTAT
   
Sequence Name 12-K120166-0022-575-G07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K120166-0022-575-G07C-8409
TATTACGTACGCAACTTCTATCTTGTAATTTTAAGAGCCAAAATTTTGGTAGTGTCAACT
TAAACAATAAACATTATTTAGACAACGAATTGAACCCTCCAGTAATAAAGGAAAACAAGA
TTATGTACCGATCATCCTTCTCATTTCCCAGACCTACCAAATCTCACTCTTTTCTTCAGT
TATAGAACAATCACGGACCTACAACAAGCAGCTACCCTTAGTTCCCTCTTACTCCCA

Last Updated on 06.12.2018 16:11