Sequences from confirmation amplicon


Confirmation sequences for line 575H12 and hit At5g37500 are shown.
Line ID 575H12
Locus At5g37500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14894675
Primers used for PCR 33fm - 8474, show primer details
   
Sequence Name 45-K111630-0022-575-H12C-33fm
GenBank Accession LN505571 [GenBank]
Sequencing primer gene specific primer, 33fm: 5'-TCGGTCCTTCTAGACAAAACCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K111630-0022-575-H12C-33fm
TAGAGTCCAACCAACAAAAAAAAACGTATTTTCTCGACAGAAACAAAATCAATATAGCTT
CAATTTCACGAGAAAGAAGAACTAAGGAAGACCAACCAAAAAATATTTTGGAAAAAAACA
GAGAAGCAGCTCTCGGGATCTTCCCGCCTTTTCTCTTCAACTTGTAAAACATTACAAGAT
CTATCTTCTACCAAAAAGACAAAGTTTCGAGTGTCAAATTCAAGAGTTGAATCGGGTATT
AAACTCGGTTTTTCTAGATCTTTGTGTCGAAAAGATCGCCGGAAATTTCATTTAACCGAT
TCTGGAAAACATGGGACGTCTCCGGAGACGGCAAGAGATAATAGATCATGAAGAAGAGGA
ATCAAACGACGACTTTTCATCAAGAAGAGGAAAACTCAGTTTAGCCGAGACGTTTCGGGG
GCTTGATTCATCAAAGCATCGGAAAATTGAAACCGATGGTCATAAGGATTATAAATACAT
CATTCATCCCAAAAACAGGTCCTTTCTTTTTTCTCTTTCAACCGAATTTATGGGCGAAAA
TACTTATTTATTTTAAAGAATATTAGGGTCATAAGGATTATAAAGAAAAGTATTTTCTAC
GTATTTATCCCAAAAACCGATGGTCATATTTATCTTTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACTTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K111629-0022-575-H12C-8409
GenBank Accession LN499349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K111629-0022-575-H12C-8409
CATTTACAATTGAAAGATAAATATGACCATCGGTTTTTGGGATAAATACGTAGAAAATAC
TTTTCTTTATAATCATTATGACCATAATATTCTTTAAAATAAATAAGTATTTTCGCCTAT
AAATACGATTGAAAGAGAAAAAAGAAAGAACCTGTTTTTGGGATGAATGATGTATTTATA
ATCATTATGACCATCGGTTTCAATTCTCCGATGCTCTGATGAATCAAGCCACCGAAACGT
CTCGGCTAAACTGAGTTTTCCTCTTCTTGATGAAACGTCGTCGTTTGATTCCTCTTCTTC
ATGATCTATTATCTCTTGCCGTCTCCGGAGACGTCCCATGTTTTCAAGAATCGGTTAAAT
GAAATCTCCGGCGATCTCTTCGACACAAAGATCTAGAAAAACAGAGTTTAATACCCGATT
CAACTCTTGAATCTGACACTCGAAACTTCGTCTTTTTGGTAGAAGATAGATCTTGTAATG
TTTTACAAGTTGAAGAGAAAAGGCGGGAAGATCACGAGAGCTGCTTCTCTGTTTTTTTCC
AAATTATTTTTTGTTTGGTCTTCTTTAGTTCTTCTTTCTCGTGATGTTAAAGCTATATTG
ATTTTGTTTCTGTCGAGAAACTACTGTTTTTTTTTTGTTTGGTTTG

Last Updated on 06.12.2018 16:11