Sequences from confirmation amplicon


Confirmation sequences for line 577A04 and hit At5g63970 are shown.
Line ID 577A04
Locus At5g63970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25608430
Primers used for PCR BE04 - 8474, show primer details
   
Sequence Name 51-K028100-022-577-A04C-8409
GenBank Accession LN507386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K028100-022-577-A04C-8409
CTTGAAAGCAAGAAATAAGAAACTAACCATCGCCAAAACCGAAACAAGGTATAAGATCAT
CTTCATCAAAGGGAGACAAAGTACGACCAATAATCGATATTGCTTTCTCATATGGATTCT
GTCTTTTACCAATGGCATGAAGGCTTTTTCTATTGAATGAATATCTCCCTGCAATGAAAA
TTCAGAACATTAACCAAAACCATTGAATCTTTTCGTAAACATGTGTAGCATTTTTTATCA
AATCAAATAATTACCTGTCCACTCATTGCTCTTGGTAAAGTCAATTCCTAGAATCAGATT
TGAAGATTCAAGTCCAGCTTCTCTCAGAGAAGTTATCACCTAAAAAGCAAAGTTCACAAG
AAATTCATCACTATGAAAGAGACTAAAATCAAGAAATAACTAAGTAAGTTTATGAGACCT
GATCCAAACTGCTGAAATGATCAGCAATATACGAAGGTTGCTGATTACGTTTATTGCTCA
TGTTCATTGAAAAAACCCTGCAGCACAAGAACCTTCAGAAATCCAATCTTTGGAGAAGAA
ACCAATCAAAATCAAGAATAAAAGATACAGTTACACAAGTGGCAAGTATATATCAACCTG
GAGTTT
   
Sequence Name 51-K028100-022-577-A04C-BE04
GenBank Accession LN507387 [GenBank]
Sequencing primer gene specific primer, BE04: 5'-TTTTGTGTTTTCAACAAACTCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K028100-022-577-A04C-BE04
GTACTGTATCTTTTATTCTTGATTTTGATTGGTTTCTTCTCCAAGATTGGATTTCTGAAG
GTTCTTGTGCTGCACGGGTTTTTTCAATGAACATGAGCAATAAACGTAATCAGCAACCTT
CGTATATTGCTGATCATTTCAGCAGTTTGGATCAGGTCTCATAAACTTACTTAGTTATTT
CTTGATTTTAGTCTCTTTCATAGTGATGAATTTCTTGTGAACTTTGCTTTTTAGGTGATA
ACTTCTCTGAGAGAAGCTGGACTTGAATCTTCAAATCTGATTCTAGGAATTGACTTTACC
AAGAGCAATGAGTGGACAGGTAATTATTTGATTTGATAAAAAATGCTACACATGTTTACG
AAAAGATTCAATGGTTTTGGTTAATGTTCTGAATTTTCATTGCAGGGAGATATTCATTCA
ATAGAAAAAGCCTTCATGCCATTGGTAAAAGACAGAATCCATATGAGAAAGCAATATCGA
TTATTGGTCGTACTTTGTCTCCCTTTGATGAAGATGATCTTATACCTTGTTTCGGTTTTG
GCGATGGTTAGTTTCTTATTTCTTGCTTTCAAGATGAATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11