Sequences from confirmation amplicon


Confirmation sequences for line 578G09 and hit At1g29370 are shown.
Line ID 578G09
Locus At1g29370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10282251
Primers used for PCR 41pq - 8474, show primer details
   
Sequence Name 49-K115259-0022-578-G09C-41pq
GenBank Accession LN506938 [GenBank]
Sequencing primer gene specific primer, 41pq: 5'-CTTTATTATCATGTGTGTATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115259-0022-578-G09C-41pq
TATTAGCACTGCTGGTTTAACGAGAGAACCGGTTCAGTAATTTTACAAATTTAAAAAGGC
AAAGAGGAAAAAAAAAGGAAAAGAAAAGTTGTTTTTTTTCTATAAGGTGAAGAAAAAATC
TGGAGAGGGTTTCGTTTTCTACAGAGAGAGATATATCTTCCCTTGAGCTTCCTTGGTGTT
CTTCGTATTGTCGTCGCGACTGAGAGAAGGCCACAAAAGCTTCGTTTATTTCCTTCAGAT
CCGATATAGTGAGAGAGAGAGAGAGAGAGAGGCAGCTTACGGCGGATAAATATGAGCAGC
AGCAAGGTCGGCGGCGGAGCAAGGAAAGGAATTCAGGATATTCCGTCGGGGTCTAGAAAA
ATAGTACAAAGCTTGACGGAAATTGTAAACTCTCCTGAGGCTGAGATCTATGCTATGCTT
AAAGAGTGCAATATGGATCCTAACGAAACCGTTAGTCGCCTCCTCTCTCAGGGTTCCTCC
TTTTTCCTCCTCCTCTATTTGTTTGAGCTGCTGTGTCTTTGGATTGTTAATTTGGGCCTG
GGGTTTTCATGTATCCCGAGGAGGATTTCTCGAAATCGACTTAATTACTAAGGGTTTCTA
TTAGGGTTTGATTGGGGTTTTTATTCTGAACAGACCAATACTTTCGTTTTTGTTAGTTTC
TAGAATTTAGTCATTGTGCAATTCTAAAGCTGTCGCCTTTAATATGGTTCCCCTTGTAAT
TGTCCAACTTTCTAGCTGGTTCGGTTTTATAGAGAAGAAAGATGTTGTTAATGGTTTGCT
TTCTTAGTTCTTGATTCAAGGCTTGTCTTTGTTCTATTATTAAGGATATTGATACTTGTT
TTTTCCTTATTCAGATCCTTTTCACGAGGTAAAGAGCCAGAAAGAAAAGAAGAAAGAGGT
GAGTCCATGTTACAAATCGTAT
   
Sequence Name 09-K115258-0022-578-G09C-8409
GenBank Accession LN490313 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115258-0022-578-G09C-8409
TATATCCTACTAGAAAGAGAAGTTGATTGTATCACACATGNGTTGAAGCATGTAATGATA
CAGAGATTGTGCCATAAGTTTGCACATAAAACGCCAACACTGGACACCAAAAGCACAAAA
CAAAACACCCCTGGGTCTATTATCCATAAGAATACGATTTGTAACATGGACTCACCTCTT
TCTTCTTTTCTTTCTTGCTCTTTACCTCGTGAAATGGATCTGAATAAGGAAAAAACAAGT
ATCAATATCATTAATAATAGAACAAAGACAAGCCTTGAATCAAGAACTAAGAAAGCAAAC
CATTAACAACATCTTTCTTCTCTATAAAACCGAACCAGCTAGAAAGTTGGACAATTACAA
GGGGAACCATATTAAAGGCGACAGCTTTAGAATTGCACAATGACTAAATTCTAGAAACTA
ACAAAAACGAAAGTATTGGTCTGTTCAGAATAAAAACCCCAATCAAACCCTAATAGAAAC
CCTTAGTAATTAAGTCGATTTCGAGAAATCCTCCTCGGGATACATGAAAACCCCAGGCCC
AAATTAACAATCCAAAGACACAGCAGCTCAAACAAATAGAGGAGGAGGAAAAAGGAGGAA
CCTTGAGAGAGGAGGCGACTAACGGTTTCGTTAGGATCCATATTGCACTCTTTAAGCATA
GCATAGATCTCAGCCTCAGGAGAGTTTACAATTTCCGTCAAGCTTTGTACTATTTTTCTA
GACCCTGACGGAATATCCTGAATTCCTTTCCTTGCTCCGCCGCCGACCTTGCTGCTGCTC
ATATTTATCCGCCGTAAGCTGCCTCTCTCTCTCTCTCTCTCTCACTATTTCGGATCTGAG
AGAAATAAACGAAGCTTTTGTGGCCTTCTCTCAGTCGCGACGACTATACGAAGAACACCA
AGGAAGCTCAAGGGAAGATATATCTCTCTCTG

Last Updated on 06.12.2018 16:11