Sequences from confirmation amplicon


Confirmation sequences for line 579D05 and hit At4g23470 are shown.
Line ID 579D05
Locus At4g23470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12250893
Primers used for PCR TN05 - 8474, show primer details
   
Sequence Name 02-K103793-0022-579-D05C-TN05
GenBank Accession LN487002 [GenBank]
Sequencing primer gene specific primer, TN05: 5'-CTCACAAGCAATTACAAACACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103793-0022-579-D05C-TN05
GCTTTTGGCTGTGTAGGGTTTCATGGTTTGCCTCAGCCAAGTGGCTTGCATATTCTCCAT
AGTTGCATGTATTGTCGGTATGGATGAGCTTTCAGAAGCTTCTCAGATACTCACATGTTG
CTCTGACATGGTGTACTGCACGTAAGAATCCCCCATCCAAAGTGCCATTCTTATAAGTTT
CGATCAAAACCCAATTTTAACCTATTGTCCTGATATATCGTACTTTGCTTGGTCTGCAGG
GTTTGCGCATGTATGCAGGTACGAGCAGTGATGATATCATATCAACCCATTCCAATCTCT
CCTTACTTAAATCCTACACTTACGGTAATATCTATAATACTTTTGTAGACACAACACAAG
ATGGAAATGGACAAGAGGGACGGTAAGTTCGGGCCACAGCCAATGGCAGTGCCTCCGGCT
CAGCAAATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATT
   
Sequence Name 10-K103794-0022-579-D05C-8409
GenBank Accession LN490622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K103794-0022-579-D05C-8409
TTGAATATATCCTGACATTTGCTGAGCCGGAGGCACTGCCATTGGCTGTGGCCCGAACTT
ACCGTCCCTCTTGTCCATTTCCATCTTGTGTTGTGTCTACAAAAGTATTATAGATATTAC
CGTAAGTGTAGGATTTAAGTAAGGAGAGATTGGAATGGGTTGATATGATATCATCACTGC
TCGTACCTGCATACATGCGCAAACCCTGCAGACCAAGCAAAGTACGATATATCAGGACAA
TAGGTTAAAATTGGGTTTTGATCGAAACTTATAAGAATGGCACTTTGGATGGGGGATTCT
TACGTGCAGTACACCATGTCAGAGCAACATGTGAGTATCTGAGAAGCTTCTGAAAGCTCA
TCCATACCGACAATACATGCAACTATGGAGAATATGCAAGCCACTTGGCTGAGGCAAACC
ATGAAACCCTACACAGCCAAAAAGCCAGCACAGATTACGAAACTTGTTTATCGTGTTTGT
AATTGCTTGTGAG

Last Updated on 06.12.2018 16:11