Sequences from confirmation amplicon


Confirmation sequences for line 579E09 and hit At3g01090 are shown.
Line ID 579E09
Locus At3g01090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:31550
Primers used for PCR TA12 - 8474, show primer details
   
Sequence Name 30-K030141-022-579-E09C-8409
GenBank Accession LN499597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K030141-022-579-E09C-8409
CTTACATTGTATAAAACTCGGGACGACAAGTATCTACTGGGATTTGCAGAGAGTACAAGG
TCCTCAGTTCTTGTTCTTGGATCTGTGTGCTGCTTTTCTTGCTCAGCTCCGAGTCCTCTG
AATACTAAACCGGACCTCTTCAAGAATAATCCCCACTTATTCCTTTTTTTTTTTTTTTCT
TATCCGCCAAAAAAATTTTTTAAAAAACGTACCCCTTTTTTTCTTCTTCTTTTTTTTTCT
TTTTCTATAGGGGGGCTCTACCCAAATCCCTTTTTTAACTTAAATGTAAAACTGACCAAA
ATTAAAAAAAAAACCTTTTGTACAAAATTGGTAGGGCATCAGGCCCCCAAAATGGATTTT
TGTTTTTGTTTCCCCCATATTTCTTAGGATAAAAACCAAAAATTTAGGGGAAAGACTTTT
TGACAAAATTTGAGGGAAAGATTTTTAATTTATTTTCTTTGAAAACAATTTACCAATTTC
AAAAGTATTTGTTTTTGTTCCG
   
Sequence Name 30-K030141-022-579-E09C-TA12
GenBank Accession LN499598 [GenBank]
Sequencing primer gene specific primer, TA12: 5'-TTGACCCATCAAATAATACACGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K030141-022-579-E09C-TA12
TCTGATTTGCTATTTGTTTTCATGAAACTAATTTAAACATCTTTCCTTCATTTTTGTCAA
AATGTCTTTCCCTTAACTTATTGATTTTAATCATAAGAAATATGGAGTAAACAAAAACAA
AAATCAATCTTGGTGGCATGATGCACTACAAGTTATGTACAAAGTGTCTTTTTTTTACTC
TTGCTCAGTCTTACATTTAAGTTAAAAGAGGTATTTGAGTAGAGCACGCATATAGAAATA
GAAAGAAAGAGAAGAAGAAAAAAAGGGTTACGTTTTCTAAATAATTTCTTAGACGAATAA
GAAAAAAAAAAAAAAAGGAATAAGTGGGGATTATTCTTAAAAAGGTCCGGTTTATTATTC
AAAGGACTCGAACCTAACCAAAAAAACCACCCCACAAATCCAAAAACAAAAACTGAGGAC
CTTGTACTCTCTGCAAATCCAGAAAAAACTTGTCCCCCCAAGTTTTATACAATTGAAAAG
GGCTTCTTGTCCGGAAAATCTCCTTGGATCACCAATGAGTATAATGGTCAATTTGGAAAA
AAAAAAAGAGTAATTCCCATTTTTTTTTCAATTCAAAAATGTAAATTCCCCCACCGTTTT
TATAAAATAAAAGTCCTTTTTGATAAAACAACAAATTACAATCCGCCGTATTTATGGGCA
AAACCAATAAACAAATTTTTCTAATTCGAAAATCTTTATTT

Last Updated on 06.12.2018 16:11