Sequences from confirmation amplicon


Confirmation sequences for line 579F06 and hit At5g05470 are shown.
Line ID 579F06
Locus At5g05470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1621313
Primers used for PCR 40sa - 8474, show primer details
   
Sequence Name 60-K116229-0022-579-F06C-40sa
GenBank Accession LN510347 [GenBank]
Sequencing primer gene specific primer, 40sa: 5'-ATAAATAGGAGCACACCCAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116229-0022-579-F06C-40sa
GGACAATCCATCAGTACCTACAGCCTTCGCCTTTCTTTCATGGCTTTCTGTAAATTCATA
ATAATAGCCAAACATCTTTAGCTAGAAGTAGAAAACAAACAGAGAAAACAAAAAAAAAAA
CCGATTAAAAAAAGGGAAAAAATTGCCTTTGTTTTGAAAAATTCCTTCAAATTGAAAACC
TTTCATTTCATTTTCACCACAATTCTTCTTGGGCTGGGGTGTCTTTCCCCTCCAAATGTC
CTTCAAAAATGCTTTTTTCATTTTTTCAAACACACCCGGAACAATTTTGTTCCCTTGTAC
AAAAAATTTTTTATAATTTTTCTTGATCAATTTAAAACATTGTTAAAAAAGGTTGCTCAG
GGAAAAAATTTTTTTTCCCTCCACCCCTTCAGGTCCATTTTTTTTAATTTCTCGGGTAAA
ACCTTCAAAAATTGATTCGGGATCATTGACTTGTAATTTTCCCACTGTTGTAATTGGTTT
CTTGTCCGGAAATTCTCCTTGGTTCACCATTGATTTTAATGGTCATTTTGGAAAAAAAAA
AAAATTATTTCCCATTTTTTTTTCATTTCAAAATT
   
Sequence Name 20-K116228-0022-579-F06C-8409
GenBank Accession LN495363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116228-0022-579-F06C-8409
AGGTAACAACACTCTTTGCCTTGAGCAGCCTTTCTCTATCATTCGTTTTAATTTGATCAT
GAAGACTTATAACATATCTTCTGTACAGGTGACCAAAGTTCGTCCCGGCTGTGTCTGAAG
AATTGAAAGATGCATTTTTTAAAGGACATTAGGAGGAGAATGACACCACAGCCAATGAAG
ATTCGTGCTGATATTGAATTGAAGTGTTTTCAGTTTGATGGAGTTTCTCCACATCAAGGT
CAGTCTTTTCCTTTTTCTTATCTGTTTTTTTTTTTGTTTCCCCGGTTTGTTTCCACCTTC
AACCAAAAAATTTTTGGCTTTTTTTATAATTTTCCGGAAACCCTTAAAAAAGGCAAAGCC
TTTAGTTCCTAATAATTTTCCATTCAAAATCAACCTCTTTCCTCCCCCCTTTTTTTTCCT
CACAACTCACCCCCTTTACAAGGTATTTTTATGGTTTTTGG

Last Updated on 06.12.2018 16:11