Sequences from confirmation amplicon


Confirmation sequences for line 579G01 and hit At3g49790 are shown.
Line ID 579G01
Locus At3g49790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18468361
Primers used for PCR NG28 - 8474, show primer details
   
Sequence Name 62-K103311-0022-579-G01C-8409
GenBank Accession LN510733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K103311-0022-579-G01C-8409
ATTTACATTGATTCATATGAAGAAGTGATGTTTTAACTCTCACAAATCTCCTTGTCTCAT
TTCCTCTCTCTCTCACTTTCTCCACATAACTCTCCACTTTACCATTTGCTCTCTCAATCA
ACACTTCTAACAACGATCTAACCGTCTCAAACGTTACCCGCCCGGTTAAATCCACCACAT
ATTTCCGGTTACTCGGTACCGATAACGACAACAACACCCGATCGATCCACGTCGCTTGTT
TCGTTGATCCAACGGTCAGCGTTTGAGACGAATCTTGACATGACGATGACCGGTTCAATT
GAACCGGTTTCCGCGACGCTCTTACAAACGTCTCAACCGCGCTGTTACATAGCGTTACAA
GCGTTTGCTTAACCTTTCCTTCA
   
Sequence Name 55-K103312-0022-579-G01C-NG28
GenBank Accession LN508780 [GenBank]
Sequencing primer gene specific primer, NG28: 5'-AAACATGAAGGAAAGGTTAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103312-0022-579-G01C-NG28
CGCGGTTGAACGTTTGTAGAGCGTCGCGGAAACCGGTTCAATTGAACCGCGTTTCGTCAT
GTCAAGATTCGTCTCAAACGCTGACCGTTGGATCAACGAAACAAGCGACGTGGATCGATC
GGGTGTCGTCGTCGTTATCGGTACCGAGTAACCGGAAATATGTGGTGGATTTAACCGGGC
GGGTAACGTTTGAGACGGTTAGATCGTTGTTAGAAGTGTTGATTGAGAGAGCAAATGGTA
AAGTGGAGAGTTATGTGGAGAAAGTGAGAGAGAGAGGAAATGAGACAAGGAGATTTGTGA
GAGTTAAAACATCACTTCTTCATATGAATCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATNTCCGCAGCGTTATA

Last Updated on 06.12.2018 16:11