Sequences from confirmation amplicon


Confirmation sequences for line 581F04 and hit At2g46310 are shown.
Line ID 581F04
Locus At2g46310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19011786
Primers used for PCR 06ih - 8474, show primer details
   
Sequence Name 38-K112875-0022-581-F04C-8409
GenBank Accession LN503042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112875-0022-581-F04C-8409
ATCCATGTGGATGAGATCAGGTTCTGTGACGAAGATGACGAACCTAAACCGGCGAGGAAA
GCGAAGAAAAAGTCCCCGGCGGCTGCGGCGGAGAACGGTGGAGATTTGGTAAAGTCTGTG
GTGAAGTATAGAGGAGTGAGACAACGACCTTGGGGAAAATTTGCGGCGGAGATTCGTGAT
CCTTCGAGTCGTACTAGACTCTGGCTTGGGACTTTTGCGACGGCGGAGGAAGCTGCTATA
GGTTACGATAGAGCCGCGATTCGAATCAAAGGTCATAACGCTCAGACGAATTTTCTCACT
CCTCCTCCTAGTCCGACGACTGAGGTGTTACCGGAAACTCCGGTGATTGACCTTGAAACT
GTCTCTGG
   
Sequence Name 54-K112876-0022-581-F04C-06ih
GenBank Accession LN508532 [GenBank]
Sequencing primer gene specific primer, 06ih: 5'-ACCAGAGACAGTTTCAAGGTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K112876-0022-581-F04C-06ih
GAGAAATTCGTCTGAGCGTTATGACCTTTGATTCGAATCGCGGCTCTATCGTAACCTATA
GCAGCTTCCTCCGCCGTCGCAAAAGTCCCAAGCCAGAGTCTAGTACGACTCGAAGGATCA
CGAATCTCCGCCGCAAATTTTCCCCAAGGTCGTTGTCTCACTCCTCTATACTTCACCACA
GACTTTACCAAATCTCCACCGTTCTCCGCCGCAGCCGCCGGGGACTTTTTCTTCGCTTTC
CTCGCCGGTTTAGGTTCGTCATCTTCGTCACAGAACCTGATCTCATCCACATGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATA

Last Updated on 06.12.2018 16:11