Genotyping and primer details


Genotyping and primer details for 581F04 - At2g46310
Line ID 581F04
Locus At2g46310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19011786 of line 581F04 Chr2:19011786
   
Insertion allele amplicon 8474 - 06ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ih: 5'-ACCAGAGACAGTTTCAAGGTCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19012150
   
wt amplicon (over InsPos) 11jm - 06ih
wt amplicon start Chr2:19011181
wt amplicon end Chr2:19012150
wt amplicon size 970 bp
Locus specific primer 06ih: 5'-ACCAGAGACAGTTTCAAGGTCAAT-3'
2nd locus specific primer 11jm: 5'-AGCTATAGAGTGAACCAAAACCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11