Sequences from confirmation amplicon


Confirmation sequences for line 582E02 and hit At2g40520 are shown.
Line ID 582E02
Locus At2g40520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16924777
Primers used for PCR 11do - 8474, show primer details
   
Sequence Name 17-K109674-0022-582-E02C-11do
GenBank Accession LN493891 [GenBank]
Sequencing primer gene specific primer, 11do: 5'-AGTTTCTGAACTCCCAGCATAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K109674-0022-582-E02C-11do
CAGATTCCAAAAGATAGAAAATATTACTTGAGATACCAATAATCATGCTTAGAAATCTCT
GGTTAATATAGTACCTTTTGTCACACTACGTCCAAGGTTGTTACTGTGTTTCAGAGGATC
CAGTATGTTGTAATATTTTACAGGAAAATATTTCCTGCTGGCTTCAACAACTCCAGTTTC
CCCAGAATATAACTCCATGCATTCTCTGAAGAACTTCTCATCTAGGAACACTTCATGATT
TCCGGTTTCTATAAATGAAGTTTAAATGTAGGTTTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K109673-0022-582-E02C-8409
GenBank Accession LN486615 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109673-0022-582-E02C-8409
GCCATTTACATTGAATTATCCTGAAACCTACATTTAAACTTCATTTATAGAAACCGGAAA
TCATGAAGTGTTCCTAGATGAGAAGTTCTTCAGAGAATGCATGGAGTTATATTCTGGGGA
AACTGGAGTTGTTGAAGCCAGCAGGAAATATTTTCCTGTAAAATATTACAACATACTGGA
TCCTCTGAAACACAGTAACAACCTTGGACGTAGTGTGACAAAAGGTACTATATTAACCAG
AGATTTCTAAGCATGATTATTGGTATCTCAAGTAATATTTTCTATCTTTTGTGTAATCTT
GCTAGGAAATATGGTACGTTTAAGAAATTGTTTTATGCTGGGATTTCAGAAAC

Last Updated on 06.12.2018 16:11