Sequences from confirmation amplicon


Confirmation sequences for line 582E03 and hit At5g58090 are shown.
Line ID 582E03
Locus At5g58090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23506142
Primers used for PCR CH42 - 8474, show primer details
   
Sequence Name 55-K106741-0022-582-E03C-CH42
GenBank Accession LN508826 [GenBank]
Sequencing primer gene specific primer, CH42: 5'-GTAGGTGCTAAATTGATGCTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K106741-0022-582-E03C-CH42
GGATGACCTTTCTTGTCACTTATAATGGGAGCTATCTGAGTACTACTTTCCCTGCGTTGA
GGAACATCCAAATTGCAATTATAACGGCTGGCCTTCAGAACCAAGTGAAGGTCACTTGTC
CCTTGAACGCTGATGTCTATGACAGCTCCACCACTTTTCCTTCTGGAGGGGACTTCAGGG
CCAATATCCGTGACCTCATGATCACTATCGTCAAGTTCTTGAGCGAAAATGGCGGTCCTT
TCACTGTTAACATCTACCCTTACATCAGTCTTTACACCAACCCCGATTTCCCAGTTGATT
ACGCCTTCTTTGACGGCAATGCACAGCCCCTGAACGATGGTGGGACTTTTTATTACAACA
TGTTTGATGCAAATTACGACACCCTAGTCCACGCGTTGGAGAAGAATGGGTTTGGTAACA
TGCCTATCATTATTGGGGAAATTGGTTGGCCTACTGATGGGGACAGTAATGCGAATTTGG
ACTACGCTAAGAAGTTCAACCATGAGTATAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTATATGTCCGCAGCGTTATTAT
   
Sequence Name 39-K106740-0022-582-E03C-8409
GenBank Accession LN503367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K106740-0022-582-E03C-8409
ACTCATGGTTGAACTTCTTAGCGTAGTCCAAATTCGCATTACCGTCACCATCAGTAGGCC
AACCAATTTCCCCAATAATGATAGGCATGTTACCAAACCCATTCTTCTCCAACGCGTGGA
CTAGGGTGTCGTAATTTGCATCAAACATGTTGTAATAAAAAGTCCCACCATCGTTCAGGG
GCTGTGCATTGCCGTCAAAGAAGGCGTAATCAACTGGGAAATCGGGGTTGGTGTAAAGAC
TGATGTAAGGGTAGATGTTAACAGTGAAAGGACCGCCATTTTCGCTCAAGAACTTGACGA
TAGTGATCATGAGGTCACGGATATTGGCCCTGAAGTCCCCTCCAGAAGGAAAAGTGGTGG
AGCTGTCATAGACATCAGCGTTCAAGGGACAAGTGACCTTCACTTGGTTCTGAAGGCCAG
CCTTTATAATTGCAATTTGGATGTTCCTCAACGCAGGGAAAGTAGTACTCAGATAGCTCC
CATTATAAGTAGACAAGAAAGGTTCATTCCCAACAGCCACATATCTGTCCAACAACCAAA
TCACAGCATCAATTTAGCACCTACAGTCGTGA

Last Updated on 06.12.2018 16:11