Sequences from confirmation amplicon


Confirmation sequences for line 583B01 and hit At1g33800 are shown.
Line ID 583B01
Locus At1g33800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12261961
Primers used for PCR 42qn - 8474, show primer details
   
Sequence Name 12-K115511-0022-583-B01C-42qn
GenBank Accession LN491716 [GenBank]
Sequencing primer gene specific primer, 42qn: 5'-CCAAATCGAAAAATCGATTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115511-0022-583-B01C-42qn
ATCTTCTCTTATCTCAGGTCATATTCATAGGTTCCTCAATCTTGATCTTGATCATCATCT
ACTTAGCAAGATCAAACATCTCTTCTTCCTCTTCAAAGCCAATCTCCAAAACAAATCTCT
CCCAAGAAGAAGAAGAAACTCAGCATAAACAAGAAGGATGTCCAACAACACAACAATGCA
CAAAGATGCCACTTTCTTTATCCGATGCATTGGTTCATTACGTCACTAGTAACGTCACTC
CACAACAAACATTTGATGAAGTCTCTGTCTCAAAGAGAGTCTTGGACAAGAAGTCTCCTT
GTAACTTCCTTGTCTTTGGCTTAGGTCACGACAGTTTAATGTGGGCATCTCTTAACCATG
GTGGTCGTACCTGTAAGTACTCTGTTTTTTCCTCTGTTTCAACAGATCTAATGAAAACTT
TCTGATTTCTCATTAAGGAAGTTTTTAAATTTCAGTGTTTATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K115510-0022-583-B01C-8409
GenBank Accession LN488046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115510-0022-583-B01C-8409
TTGAATAAACACTGAAATTTAAAACTTCCTTAATGAGAAATCAGAAAGTTTTCATTAGAT
CTGTTGAAACAGAGGAAAAAACAGAGTACTTACAGGTACGACCACCATGGTTAAGAGATG
CCCACATTAAACTGTCGTGACCTAAGCCAAAGACAAGGAAGTTACAAGGAGACTTCTTGT
CCAAGACTCTCTTTGAGACAGAGACTTCATCAAATGTTTGTTGTGGAGTGACGTTACTAG
TGACGTAATGAACCAATGCATCGGATAAAGAAAGTGGCATCTTTGTGCATTGTTGTGTTG
TTGGACATCCTTCTTGTTTATGCTGAGTTTCTTCTTCTTCTTGGGAGAGATTTGTTTTGG
AGATTGGCTTTGAAGAGGAAGAAGAGATGTTTGATCTTGCTAAGTAGATGATGATCAAGA
TCAAGATTGAGGAACCTATGAATATGACCTTGAGATTAAGAGAAGATGGAGATTTGGTCC
TCATTTTTTCTGACTAAAGAATCGATTTTTCGATTTGG

Last Updated on 06.12.2018 16:11