Sequences from confirmation amplicon


Confirmation sequences for line 583E05 and hit At5g20540 are shown.
Line ID 583E05
Locus At5g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6949399
Primers used for PCR 18lu - 8474, show primer details
   
Sequence Name 05-K114086-0022-583-E05C-18lu
GenBank Accession LN488441 [GenBank]
Sequencing primer gene specific primer, 18lu: 5'-AATTTGATTACAGGACAAAGACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114086-0022-583-E05C-18lu
AATTCTTTATTTATAGAAAACGTTAAAAATTATTGGTGAAAATGATTTAATATAACATGT
ACCAAACAATGATTTGGAATGTTTTATTAAACAAAATAAATAAGGTTCGTTACATTCGTA
AACATCTAACAACTCCATAAAGATTTAAGTTAGTAGCAAACTTATCAGACACATTCTAGA
GTGATTTCATACACTATGTATGACTGATAATTCATAGGTTTAACCAAAGTTTGAAGAAAA
CAAAAAGAGGAGGAAAAAATGATTATCAGTCAAATAAGTTTATATTGAGAAAAGCAAAAA
AAAAAAAAAAACAAAGGGAAAAAATTTCCCCCCAAAAGCCTTTAAAAAAAAAAAACCCCC
ATTTTGTTTTTTGGACCTTTTAGGGCCTTTGTTTTCTATTGGGGGGAAAAGTTTATTGGC
AAAAACTTTTCCCCCATTCCCCTTTTTTTTTACCCCACTTTTTTTCCCATTTTCCCCCCC
GGGAATTTTTCAATTGGAAAGGGGTTCCGGGCCGGGAAATTCCCCGGGATCCCCCAGGGA
TTGGAGGGGCAATTGGGGAAAAAAAAAAAAAGAATTCCCATTTTTTTTTCAATTCAAAAA
GGGAAATTCCCCCCCGCTTTTAAAA
   
Sequence Name 29-K114085-0022-583-E05C-8409
GenBank Accession LN499383 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114085-0022-583-E05C-8409
TTGAATATATCCTGAGTGTGAAAATGGGAAAGAAAGTAGGTAAAAATAAAGAGAGAATGG
GGACAGAGTTGTCGACAGTAACGCTTCACGCCTAATGAGAAGCAAAGGCCGTAAAAGGTC
CAAAAAGCAAAATTGGGTGTCTTCTTTTTTAAGTGCTTTCTGGGGTCATCTCTTCTCATT
CTGTCTTTTTTTTTTTTTGCTTTTCCCAAAAAAAACTTTTTTGACGGAAAAACTTTTTTT
CCCCCCCTTTTTTTTTTTCTCAAACTTTGGTTAAACCTTAAAATTTTCCTTCAAACAATG
GGGAAAAAAACCCCCCAAAAGGGTTTTAAAAATTTTGCCACAAAATTAAATTTTTTGGGG
TTTTTTAAATTTTTAAAAATGGAACAAACCTTTTTTTTTTTTTTTAAAAAAAAATTCCAA
AACATTTTTGGGGAACGTTTAAATAAAAACTTTTTCCCCAAAAATTTTTAACTTTTTTTA
AAAAAAAAAAAAATTTTTTTTTATTGATTCCTTATTCCCCCCCTTTTTT

Last Updated on 06.12.2018 16:11