Sequences from confirmation amplicon


Confirmation sequences for line 584E02 and hit At5g65180 are shown.
Line ID 584E02
Locus At5g65180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26047165
Primers used for PCR 19vi - 8474, show primer details
   
Sequence Name 70-K117914-0022-584-E02C-19vi
GenBank Accession LN512458 [GenBank]
Sequencing primer gene specific primer, 19vi: 5'-AAATTATCAAGTGGAGGTGTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117914-0022-584-E02C-19vi
ATGAAGAGACAGAGATGAACAAATGCAAGTCTGCCGTTAGACGTATCAGAAAGATGGAGA
AAGATGTTGAAGATGCTTGCTCCACTGGTATAAATTTAGCTAATATCTTTCTCTCTTAGT
TGGGGATTATCAAACACATTCTGGCTCTCAATCTTTGATTGTCTTCAGCAAAAGACCCGA
GGAAAGAATCATTGGCAAAAGAATTGGAAGAGGAAGAAAACATTCTGAGACAATCTGTTG
AGAAACTTAAATCTGTTGAAGAAAGTAGGACATCTCTCGTAAATCATCTTAGGGAAGCGC
TACGGGAACAGGTAAAGGTCTTGCAATCTTGCAAGTTCAATAATTTGCTCAGACATTTCA
ATTACTTACTGAGTTTTGTGTATCAGGAATCTGAATTGGAGAATCTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACATTATA
   
Sequence Name 30-K117913-0022-584-E02C-8409
GenBank Accession LN499901 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117913-0022-584-E02C-8409
TTGAAGATTCTCCAATTCAGATTCCTGATACACAAAACTCAGTCATTTAATTGAAATGTC
TGAGCAAATTATTGAACTTGCAAGATTGCAAGACCTTTACCTGTTCCCGTAGCGCTTCCC
TAAGATGATTTACGAGAGATGTCCTACTTTCTTCAACAGATTTAAGTTTCTCAACAGATT
GTCTCAGAATGTTTTCTTCCTCTTCCAATTCTTTTGCCAATGATTCTTTCCTCGGGTCTT
TTGCTGAAGACAATCAAAGATTGAGAGCCAGAATGTGTTTGATAATCCCCAACTAAGAGA
GAAAGATATTAGCTAAATTTATACCAGTGGAGCAAGCATCTTCAACATCTTTCTCCATCT
TTCTGATACGTCTAACGGCAGACTTGCATTTGTTCATCTCTGTCTCTTCATTTGAGTTCT
CTGCACGAACCAAATTGAATGCAGATACTATCTTCTCGGACACACCTC

Last Updated on 06.12.2018 16:11