Sequences from confirmation amplicon


Confirmation sequences for line 585A06 and hit At1g35170 are shown.
Line ID 585A06
Locus At1g35170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12870792
Primers used for PCR 20oc - 8474, show primer details
   
Sequence Name 36-K114720-0022-585-A06C-20oc
GenBank Accession LN502329 [GenBank]
Sequencing primer gene specific primer, 20oc: 5'-TAATATTATGGCGGGAGAATTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114720-0022-585-A06C-20oc
CACATTTTTCTCGGTGGCCCGAATGGTGGGTGGACCTTACCTAGTTTACGTAACAATACC
AGCTGATAATCCCATCCTCATCAAGGTATACAGTATCTTAATAGATGAATATGATTTACA
TTTCGTTCTAGGCTATACTTATTATTGTTTCATCAATTGTTCATAGTTTTATAAAAATGT
GAAACTTTCAAAACTGAAAAGAAGATTTTGTTTAGGAAAAGATGCTAAAATATTTTTCAT
GATCACAAATTTCAGTTGAGATATAACATTTCATGTGCGTTTTTTTGTGTTCTTATTTTC
TTAGGTAATGGGGTTGGGATTACAACTTGTGAGCGCGTTCTATAAATTACTCAAAATACC
AATACATTACACTAAAAAATCTACAAATACTGTATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 16-K114719-0022-585-A06C-8409
GenBank Accession LN493535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114719-0022-585-A06C-8409
TTTGAGTAATTTATAGAACGCGCTCACAAGTTGTAATCCCAACCCCATTACCTAAGAAAA
TAAGAACACAAAAAAACGCACATGAAATGTTATATCTCAACTGAAATTTGTGATCATGAA
AAATATTTTAGCATCTTTTCCTAAACAAAATCTTCTTTTCAGTTTTGAAAGTTTCACATT
TTTATAAAACTATGAACAATTGATGAAACAATAATAAGTATAGCCTAGAACGAAATGTAA
ATCATATTCATCTATTAAGATACTGTATACCTTGATGAGGATGGGATTATCAGCTGGTAT
TGTTACGTAAACTAGGTAAGGTCCACCCACCATTCGGGCCACCGAGAAAATTGTTGCAAA
AAACACCTCCACAAAATAATGATTATTATTACGTAATTCTCCC

Last Updated on 06.12.2018 16:11