Sequences from confirmation amplicon


Confirmation sequences for line 585E07 and hit At5g41700 are shown.
Line ID 585E07
Locus At5g41700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16677024
Primers used for PCR JV50 - 8474, show primer details
   
Sequence Name 23-K101325-0022-585-E07C-8409
GenBank Accession LN496486 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101325-0022-585-E07C-8409
GCCATTTACAATTGAATATTACTACATACACAAACATTCAAGAAGCAAAGCTTGTGCATT
GAGCAAAATCCTAGCTGAAAATGTAGATGCTCTTGCTATACTTTTGGAACATAAACGAAC
ATTGGAGAACCGATGTTTCTGAAAAATGAGAGTCACCTTGGAAATGGTGAGGGCAGGGCT
CCATTGTTCTTTCAAAATGTCAAGGCAAATGCTTCCGTTGCTGTTGATATTAGGGTGAAA
CACCTTCGTCCTAAATGCAACCTGCAAATAAAAACAAAACAACCAATAAAAGTGTCAAGA
TTAAGCCAAGAAATTGATTTGATTTGGGGGCAAATACAAGTTTTAATTAGGTCCAAGAAT
TTCAAGGAACTCTACTAAACCTAAAGTTCGTGGTAATCTAAGAGTTGTTCCAATATTGAA
TACCTTTGGTGGTTTGAATGGATAGTCCGGAGGGAAGTGAATGGTAACGAGAAACACACC
GCCTGAATACGGACTCTCTGCAGGACCCATAATTGTAGCTTGCCAATGAAACATGTCTTC
AGCAACTGGACCTGCAAAAACTAAAACATAAGCAAAAAAAACACCACANACCAAAG
   
Sequence Name 23-K101331-0022-585-E07C-JV50
GenBank Accession LN496487 [GenBank]
Sequencing primer gene specific primer, JV50: 5'-CTAGAATCCTTCTTTGGTTTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101331-0022-585-E07C-JV50
TTTTTGCAGTCCGTTGCTGAAGACTGTTTCATTGGCAAGCTACAATTATGGGTCCTGCAG
AGAGTCCGTATTCAGGCGGTGTGTTTCTCGTTACCATTCACTTCCCTCCGGACTATCCAT
TCAAACCACCAAAGGTATTCAATATTGGAACAACTCTTAGATTACCACGAACTTTAGGTT
TAGTAGAGTTCCTTGAAATTCTTGGACCTAATTAAAACTTGTATTTGCCCCCAAATCAAA
TCAATTTCTTGGCTTAATCTTGACACTTTTATTGGTTGTTTTGTTTTTATTTGCAGGTTG
CATTTAGGACGAAGGTGTTTCACCCTAATATCAACAGCAACGGAAGCATTTGCCTTGACA
TTTTGAAAGAACAATGGAGCCCTGCCCTCACCATTTCCAAGGTGACTCTCATTTTTCAGA
AACATCGGTTCTCCAATGTTCGTTTATGTTCCAAAAGTATAGCAAGAGCATCTACATTTT
CAGCTAGGATTTTGCTCAATGCACAAGCTTTGCTTCTTGAATGTTTGTGTATGTAGTAAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGAT
GTCCGCA

Last Updated on 06.12.2018 16:11