Sequences from confirmation amplicon


Confirmation sequences for line 587H06 and hit At2g29390 are shown.
Line ID 587H06
Locus At2g29390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12612325
Primers used for PCR 27nt - 8474, show primer details
   
Sequence Name 54-K114572-0022-587-H06C-27nt
GenBank Accession LN508558 [GenBank]
Sequencing primer gene specific primer, 27nt: 5'-AATGAGAACACTGACAACTTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114572-0022-587-H06C-27nt
AGCTATTAGTTAGAAGACCACTGAATCATGAGAAGAAAAGACAAACCTGAATTTTGTAGT
TGCTCAGAAAACCAGTCCTTTCTAGGAAAATGTAAGGGAGTCCAGACAAGAAAAACACGC
TTTCATGAAGTATAAAACTCCCAATACACGCCAGTTGAAAGTCACTGAAATGCGTAACAA
GGTACTGCATCATCGCCACAAAGAAGGGTCAGTGTCACAGCTAGAACTGTCAAACTAAAG
AGCTAAACATGATTGCCAAAGGGAGATAAAATTTGTTCAATTCCACGAAAACTGAAGGCA
ATTGTATATCATTGACACCATCCATCCAGTCACTCACGCAGAANAGCAAGTTAGGAAGAC
GTTCAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATG
   
Sequence Name 42-K114571-0022-587-H06C-8409
GenBank Accession LN504554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114571-0022-587-H06C-8409
TTACATTGAATATATCCTTGAACGTCTTCCTAACTTGCTTTTCTGCGTGAGTGACTGGAT
GGATGGTGTCAATGATATACAATTGCCTTCAGTTTTCGTGGAATTGAACAAATTTTATCT
CCCTTTGGCAATCATGTTTAGCTCTTTAGTTTGACAGTTCTAGCTGTGACACTGACCCTT
CTTTGTGGCGATGATGCAGTACCTTGTTACGCATTTCAGTGACTTTCAACTGGCGTGTAT
TGGGAGTTTTATACTTCATGAAAGCGTGTTTTTCTTGTCTGGACTCCCTTACATTTTCCT
AGAAAGGACTGGTTTTCTGAGCAACTACNAAATTCAGGTTTGTCTTTTCTTCTCATGATT
CAGTGGTCTTCTAACTAATAGCTTTGTGTGTCTGTTCAAATATAAACTGAATGGAAAGAT
ATCACCAAGTTGTCATGATTCTCAT

Last Updated on 06.12.2018 16:11