Sequences from confirmation amplicon


Confirmation sequences for line 588F04 and hit At1g07900 are shown.
Line ID 588F04
Locus At1g07900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2442967
Primers used for PCR 42tv - 8474, show primer details
   
Sequence Name 31-K117118-0022-588-F04C-42tv
GenBank Accession LN500318 [GenBank]
Sequencing primer gene specific primer, 42tv: 5'-CGTAGAGAAATTTAGTAGCCACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117118-0022-588-F04C-42tv
CAAAAGCCACATCATAATTCGAGCTTGTGATGTCAGGGTGGTCACCTCCTTCCCTACATC
ATTTTGTGTGTCTCCCCTATATATACACTTCCCCACCAAAAAGCACTTCACCTCTCAAAG
ACTCTACTTACACATTTCTCATCTTCCTCCCCATAATTCACAGTTTTTGCGTTGGTATGG
AGAGTAAAAGTGACGCTTCTGTCGCCACCACTCCAATCATATCTTCTTCCTCTTCTCCTC
CACCATCACTTTCACCGCGGGTAGTGCTTAGTCCATGTGCTGCTTGCAAAATCTTGAGGC
GACGTTGTGCAGAGAGATGCGTTTTGGCACCGTACTTCCCTCCAACGGATCCGGCCAAGT
TCACTATTGCACACCGCGTCTTTGGAGCCAGCAACATCATTAAGTTCTTGCAGGTACCTA
AATTATCTATATATCTATGGGTGTAATAACTATAAGCTCTTTAGTTCTTAGAAAGAATTA
CATTAAGAAAGACCCGATTTTATATTAATGATATAGATGGATGCAATAACTATAGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTATTATAAG
GATACCCTCGGGTCTGTCTGGATGCCACGAGTGAAAAGAAAGTTGGATCTACATTGTATC
TCACCTGA
   
Sequence Name 09-K117117-0022-588-F04C-8409
GenBank Accession LN490367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117117-0022-588-F04C-8409
ATAGTTATTGCATCCATCTATATCATTAATATAAAATCNGGGTCTTTCTTAATGTAATTC
TTTCTAAGAACTAAAGAGCTTATAGTTATTACACCAATAGATATATAGATAATTTAGGTA
CCTGCAAGAACTTAATGATGTTGCTGGCTCCAAAGACGCGGTGTGCAATAGTGAACTTGG
CCGGATCCGTTGGAGGGAAGTACGGTGCCAAAACGCATCTCTCTGCACAACGTCGCCTCA
AGATTTTGCAAGCAGCACATGGACTAAGCACTACCCGCGGTGAAAGTGATGGTGGAGGAG
AAGAGGAAGAAGATATGATTGGAGTGGTGGCGACAGAAGCGTCACTTTTACTCTCCATAC
CAACGCAAAAACTGTGAATTATGGGGAGGAAGATGAGAAATGTGTAAGTAGAGTCTTTGA
GAGGTGAAGTGCTTTTTGGTGGGGAAGTGTATATATAGGGGAGACACACAAAATGATGTA
GGGAAGGAGGTGACCACCCTGACATCACAAGCTCGAATTATGACGTGGCTTTTTTGCTAA
TTGAAAGTTGAAACATATTTCCACGTAACGGTGGCTACTAAATTTCTCTA

Last Updated on 06.12.2018 16:11